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関連する概念動画

Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Types of RNA01:20

Types of RNA

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
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Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

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ノンコーディングRNAと遺伝子サイレンシング

Mikel Zaratiegui1, Danielle V Irvine, Robert A Martienssen

  • 1Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.

Cell
|February 27, 2007
PubMed
まとめ
この要約は機械生成です。

非コーディングRNAは,配列特異の相互作用を通じて遺伝子発現を調節する. このレビューでは,ヘテロクロマチンの形成や転置可能な元素の制御などの静止プロセスにおけるそれらの役割を調査します.

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Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
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Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

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Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
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Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

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Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
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科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • エピジェネティクス エピジェネティクス

背景:

  • 非コーディングRNAは,遺伝子発現における規制的な役割としてますます認識されています.
  • 遺伝子サイレンシングのメカニズムは,細胞のプロセスと発達に不可欠です.

研究 の 目的:

  • 様々な遺伝子サイレンシング経路におけるノンコーディングRNAの多様な役割を検討する.
  • 非コーディングRNA媒介遺伝子調節の基礎となるメカニズムについて議論する.
  • 異なるサイレンシング現象の間の類似点を強調する.

主な方法:

  • 遺伝子サイレンシングにおけるノンコーディングRNA機能に関する文献レビュー.
  • RNAの干渉とコトランスクリプション処理を含むメカニズムの分析.
  • 異なる生物学的文脈における静音化の比較研究.

主要な成果:

  • 非コーディングRNAは,ヘテロクロマチン,転置可能な元素,および発達的に切断されたDNAの静止に関与しています.
  • RNAの干渉やその他のメカニズムは,コトランスクリプション処理を媒介する.
  • 非コーディングRNAサイレンシングとインプリントとX不活性化のようなプロセスには類似点がある.

結論:

  • 非コーディングRNAは,静止クロマチンの状態を確立し維持する上で重要な役割を果たします.
  • 非コーディングRNAによる遺伝子サイレンシングは,RNAレベルでの相互作用を含み,規制配列と潜在的に関係しています.
  • 非コーディングRNAの機能を理解することは,表遺伝的調節とゲノム安定性についての洞察を提供します.