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関連する概念動画

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.

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関連する実験動画

Updated: Jul 16, 2026

Preparation of Meiotic Chromosome Spreads from Mouse Oocytes for Assessment of Synapsis and Recombination
09:24

Preparation of Meiotic Chromosome Spreads from Mouse Oocytes for Assessment of Synapsis and Recombination

Published on: July 18, 2025

アナファーゼ発現は,DNA複製が完了する前に,チェックポイント応答が完結した状態で発生します.

Jordi Torres-Rosell1, Giacomo De Piccoli, Violeta Cordon-Preciado

  • 1Cell Cycle Group, Medical Research Council (MRC) Clinical Sciences Centre, Faculty of Medicine, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, UK.

Science (New York, N.Y.)
|March 10, 2007
PubMed
まとめ

細胞のチェックポイントは,停滞したDNA複製フォークでミトーシスを防止します. しかし,この研究では,Smc5-Smc6複合体は,既知のチェックポイントとは関係なく,細胞分裂前の複製を完了するために重要であることが示されています.

さらに関連する動画

Detection of DNA Double-Stranded Breaks in Mouse Oocytes
07:46

Detection of DNA Double-Stranded Breaks in Mouse Oocytes

Published on: June 23, 2023

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

関連する実験動画

Last Updated: Jul 16, 2026

Preparation of Meiotic Chromosome Spreads from Mouse Oocytes for Assessment of Synapsis and Recombination
09:24

Preparation of Meiotic Chromosome Spreads from Mouse Oocytes for Assessment of Synapsis and Recombination

Published on: July 18, 2025

Detection of DNA Double-Stranded Breaks in Mouse Oocytes
07:46

Detection of DNA Double-Stranded Breaks in Mouse Oocytes

Published on: June 23, 2023

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

科学分野:

  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは

背景:

  • 細胞のチェックポイントは通常,DNA複製フォークが停止した場合,ミトーシスを停止します.
  • チェックポイントがミトーシス前にDNA複製の完成を保証するかどうかは不明です.

研究 の 目的:

  • ミトーシス前に細胞のチェックポイントによってDNA複製の完了が監視されているかどうかを調査する.
  • Smc5-Smc6複合体の複製と細胞分裂における役割を理解する.

主な方法:

  • イーストのsmc5-smc6変異体を使ってDNA複製とミトーシスを研究した.
  • リボソームDNA遺伝子クラスターを含む自然レプリケーションを阻害する場所での複製タイミングを分析した.
  • 機能的なSmc5-Smc6.6がない場合の研究された染色体分離.

主要な成果:

  • smc5-smc6変異体では,特にリボソームDNAの位置において,複製が著しく遅れた.
  • ミトスの侵入は,smc5-smc6変異体における未完成の複製で発生し,染色体非分裂につながった.
  • smc5-smc6変異体における複製フォークの障害を取り除くことで,複製と分離の間の時間的な結合が回復しました.

結論:

  • Smc5-Smc6複合体は,ミトーシス前のDNA複製のタイムリーな完成に不可欠です.
  • 既知の細胞チェックポイントは,DNA複製の完了を監視していないようです.
  • 複製完成と染色体分離は,機能的なSmc5-Smc6.6が欠けているsmc5-smc6変異体では一時的に切り離されている.