ヒスティジンの銅リガンドのプロトネーションは,フェルンのプラストオシアニンに含まれています
Rinske Hulsker1, Annabelle Mery, Ellen A Thomassen
1Leiden Institute of Chemistry, Leiden University, Gorlaeus Laboratories, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.
Journal of the American Chemical Society
|March 21, 2007
まとめ
プラストオシアニン (Plastocyanin) とは
科学分野:
- バイオケミストリー バイオケミストリー
- 光合成に関する研究.
- タンパク質の構造-機能分析
背景:
- プラストオシアニンは,光合成における重要な電子伝達物質である.
- 低pHは,ヒスティジンのプロトネーション経由でプラストオシアニン・レドックス活性に影響する.
- ファーンのプラストオシアニンのHis90プロトネーションは,以前は存在しないと考えられていた.
研究 の 目的:
- 低pHでフェルンプラストオシアニンのHis90プロトネーションを調査する.
- プロトネーションを防止するPhe12とGly36の役割について,テストが提案された.
- 溶液と結晶構造データの不一致を明らかにする.
主な方法:
- 再結合タンパク質の発現と浄化.
- 核磁共振 (NMR) スペクトロスコーピー. 核磁共振 (NMR) スペクトロスコーピー. 核磁共振 (NMR) スペクトロスコーピー. 核磁共振 (NMR) スペクトロスコーピー.
- X線結晶学とサイクル電圧計.
主要な成果:
- 野生型のフェルンプラストオシアニンは,溶液中のHis90プロトネーション (pKa 4.4) を表しています.
- Phe12LeuとGly36Proの変異は,陽子化の行動を変化させなかった.
- クリスタル構造はHis90プロトネーションを明らかにせず,クリスタル接触干渉を示唆しました.
結論:
- フェルンのプラストオシアニンのHis90プロトネーションは,以前の報告とは対照的に,溶液で発生します.
- Phe12とGly36の相互作用は,His90のプロトネーションを防ぐことはできません.
- 結晶学は,フェルンのプラストオシアニンプロトネーションの溶液の振る舞いを正確に反映しない可能性があります.
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