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Updated: Jul 16, 2026

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
08:37

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

Published on: November 5, 2014

ミニチュアエステラゼの協同阻害に基づく生理学的条件下での光ADPセンシング

Laurent Vial1, Pascal Dumy

  • 1Département de Chimie Moléculaire, UMR-5250, ICMG FR-2607, CNRS--Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France.

Journal of the American Chemical Society
|April 3, 2007
PubMed
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No abstract available in PubMed .

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A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

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Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ06:03

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...