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Ssn6-Tup1は酵母における転写の一般的な抑制剤である
C A Keleher1, M J Redd, J Schultz
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Cell
|February 21, 1992
まとめ
酵母菌Saccharomyces cerevisiaeの細胞タイプは,α2およびMcm1タンパク質を必要とします. しかし,Ssn6およびTup1タンパク質は,一般的な抑制機構として作用し,転写抑制に不可欠です.
科学分野:
- 分子生物学は分子生物学である.
- イースト遺伝学 イースト遺伝学
- トランスクリプションに関する規則
背景:
- ホメオドメインタンパク質アルファ2とSRF型タンパク質Mcm1は,Saccharomyces cerevisiaeの細胞型決定に不可欠である.
- これらのタンパク質は,特定のDNAオペレータと結合し,転写抑制のための遺伝子を標的にします.
研究 の 目的:
- アルファ2-Mcm1.1.によって媒介される転写抑制のメカニズムを調査する.
- この抑圧過程におけるSsn6およびTup1タンパク質の役割を決定する.
主な方法:
- アルファ2-Mcm1オペレーターの占有率だけでは抑圧には不十分であることを示す.
- これは,Ssn6 (TPRタンパク質) とTup1 (β-トランスデューシン重複タンパク質) が抑制に必要であることを示しています.
- バクテリアのDNA結合ドメインを利用して,Ssn6をプロモーターに勧誘する.
主要な成果:
- 転写抑制には,Ssn6とTup1の両方の存在が必要です.
- Ssn6は,プロモーターをターゲットにすると転写を抑制することができ,Tup1は,この活動に不可欠です.
- アルファ2-Mcm1複合体は直接的に抑制を引き起こすのではなく,Ssn6-Tup1.1.を通じてそれを促進する.
結論:
- Ssn6-Tup1は酵母における一般的な転写抑制剤として機能する.
- この抑制複合体は,アルファ2-Mcm1.1.を含む様々な配列特異のDNA結合タンパク質によってプロモーターを標的として採用されます.
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