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Updated: May 12, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

RNAポリメラーゼIIの遠隔部位間の通信は,ユビキチル化因子とポリメラーゼCTDを介して行われる

Baggavalli P Somesh1, Stefan Sigurdsson, Hideaki Saeki

  • 1Mechanisms of Transcription Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, UK.

Cell
|April 10, 2007
PubMed
まとめ

転写停止は,RNAポリメラーゼII (RNAPII) の至る所に存在することを引き起こします. Rsp5 ダイマーと Ubc5 を含むカップリングされた改変メカニズムは,延伸と DNA 修復の間にRNAPII 普遍性を説明します.

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Eukaryotic RNA Polymerases00:58

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Eukaryotic RNA Polymerases00:58

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RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
RNA Polymerase II Accessory Proteins02:36

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Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...