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ニューロン経路NALCNは,静止中のナトリウム透過性に寄与し,正常な呼吸器リズムに必要なものです
Boxun Lu1, Yanhua Su, Sudipto Das
1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Cell
|April 24, 2007
まとめ
タンパク質NALCNは,神経系の興奮性に欠かせない重要なナトリウム (Na+) の漏れ伝導性を形成します. この発見は,NALCNCNを特定するものである.
科学分野:
- 神経科学は神経科学である.
- 分子生物学は分子生物学である.
- イオンチャンネル生理学 イオンチャンネル生理学
背景:
- 神経系の基礎刺激性は,静止状態の"漏れ"のNa+の浸透性によって決定される.
- テトロドトキシン (TTX) とセシウム (Cs+) 耐性Na+のリーク伝導性の分子同一性は不明である.
研究 の 目的:
- TTXとCs+に耐性のあるNa+のリーク伝導性を有する分子成分を特定する.
- この伝導性の神経機能と生存における生理学的役割を解明する.
主な方法:
- NALCN変異マウスを利用して,NALCNタンパク質の機能を調査しました.
- 電気生理学的記録 (脳幹・脊髄,海馬の神経) を実施し,神経発火と膜潜在力を評価した.
- 変異性ニューロンにおける静止膜ポテンシャルに対する細胞外Na+濃度の影響を分析した.
主要な成果:
- Na+の漏れ伝導量の源として,新しい電圧独立で非選択的なカチオンチャネルであるNALCNを特定しました.
- NALCN変異マウスは,呼吸リズムが乱れ,生後死亡率を示しています.
- TTXとCs+に抵抗するNa+漏れ電流と,NALCN変異ニューロンにおける変異した静止膜ポテンシャルがない.
結論:
- NALCNは,ニューロンの興奮性を制御するために重要な背景のNa + リーク伝導性を形成します.
- この発見は,NALCNが呼吸制御と神経機能における重要な役割を明らかにしています.
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Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
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01:15Resting Potential Decay
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Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
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01:24Non-gated Ion Channels
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
01:26Voltage-gated Ion Channels
Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...