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Protein Folding01:22

Protein Folding

Overview
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Protein Folding01:25

Protein Folding

Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Polymer Classification: Stereospecificity01:26

Polymer Classification: Stereospecificity

Polymerization generates chiral centers along the entire backbone of a polymer chain. Accordingly, the stereochemistry of the substituent group has a significant effect on polymer properties. Polymers formed from monosubstituted alkene monomers feature chiral carbons at every alternate position in the polymer backbone. Relative to the predominant orientation of substituents at the adjacent chiral carbons, the polymer can exist in three different configurations: isotactic, syndiotactic, and...
Cationic Chain-Growth Polymerization: Mechanism00:57

Cationic Chain-Growth Polymerization: Mechanism

The cationic polymerization mechanism consists of three steps: initiation, propagation, and termination. In the initiation step of the polymerization process, the π bond of a monomer gets protonated by the Lewis acid catalyst, which is formed from boron trifluoride and water. The protonation of the π bond generates a carbocation stabilized by the electron‐donating group. In the propagation step, the π bond of the second monomer acts as a nucleophile and attacks the generated carbocation,...
[3,3] Sigmatropic Rearrangement of Allyl Vinyl Ethers: Claisen Rearrangement01:24

[3,3] Sigmatropic Rearrangement of Allyl Vinyl Ethers: Claisen Rearrangement

The Claisen rearrangement is a [3,3] sigmatropic rearrangement of allyl vinyl ethers to unsaturated carbonyl compounds. The rearrangement is a concerted pericyclic reaction proceeding via a chair-like transition state.

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Updated: Jun 25, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

エラスティン型ポリペプチドにおける力によるプロリルシス・トランス・イソメリゼーション.

Alexei Valiaev1, Dong Woo Lim, Terrence G Oas

  • 1Department of Mechanical Engineering and Materials Science, and Center for Biologically Inspired Materials and Materials Systems, Duke University, Durham, NC 27708, USA.

Journal of the American Chemical Society
|May 2, 2007
PubMed
まとめ

単分子力スペクトロスコピーは,力によって誘発されたプロリルシス-トランスイソメリゼーションにより,エラスティン型ポリペプチド (ELP) が温度独立の延長を経験することを明らかにしました. この発見は,ポリペプチドにおけるこの重要な形状の変化を研究するための新しい方法を提供します.

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Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry
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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

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Last Updated: Jun 25, 2026

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14:55

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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Published on: February 3, 2022

科学分野:

  • バイオフィジックス 生物物理学
  • ポリマーサイエンス (Polymer Science) とは,ポリマーサイエンス (Polymer Science) とは,ポリマーサイエンス (Polymer Science) とは,ポリマーサイエンス (Polymer Science) とは,ポリマーサイエンス (Polymer Science) とは,ポリマーサイエンス (Polymer Science) とは,
  • 構造生物学 構造生物学とは

背景:

  • エラスティン型ポリペプチド (ELP) は,繰り返しVPGXG配列を持つ刺激反応性ポリマーである.
  • ELPの構成メカニズムは,通常,様々な溶媒条件下で研究されます.

研究 の 目的:

  • ELPの力-拡張曲線で観察された説明できない拡張的移行を調査する.
  • ELPにおける力による形状変化の背後にある分子メカニズムを特定する.

主な方法:

  • 単分子力スペクトロスコーピー (SMFS) は,力拡張曲線を測定する.
  • ポリ (L-プロリン) とポリ (L-ライシン) を用いたコントロール実験.
  • 弾力的に結合された2状態モデルを用いたモンテカルロシミュレーション.

主要な成果:

  • 標準的ポリマーモデルで説明できない,ELPの温度に依存しない拡張変遷を観測した.
  • 延長の原因として,力によって誘発されたペプチジルプロリルシストランスイソメリゼーションを特定しました.
  • 同様の変異は,ポリ (L-プロリン) でも,ポリ (L-ライシン) でも示されていない.
  • ヒステレシス実験では,シスプロリン状態の枯渇が示唆された.

結論:

  • 力の作用によるプロリルシストランスイソメリゼーションは,ELPの形状の変化における重要なメカニズムです.
  • SMFSは,ポリペプチドにおけるプロリルシストランスイソメリゼーションを測定するために利用できます.
  • この研究は,プロリンを含むポリペプチドの力誘発プロリルシストランスイソメリゼーションの最初の実証を提供します.