Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Predicting Molecular Geometry02:27

Predicting Molecular Geometry

VSEPR Theory for Determination of Electron Pair Geometries
Hybridization of Atomic Orbitals I03:24

Hybridization of Atomic Orbitals I

The mathematical expression known as the wave function, ψ, contains information about each orbital and the wavelike properties of electrons in an isolated atom. When atoms are bound together in a molecule, the wave functions combine to produce new mathematical descriptions that have different shapes. This process of combining the wave functions for atomic orbitals is called hybridization and is mathematically accomplished by the linear combination of atomic orbitals. The new orbitals that...
Valence Bond Theory02:42

Valence Bond Theory

Coordination compounds and complexes exhibit different colors, geometries, and magnetic behavior, depending on the metal atom/ion and ligands from which they are composed. In an attempt to explain the bonding and structure of coordination complexes, Linus Pauling proposed the valence bond theory, or VBT, using the concepts of hybridization and the overlapping of the atomic orbitals. According to VBT, the central metal atom or ion (Lewis acid) hybridizes to provide empty orbitals of suitable...
Newman Projections02:06

Newman Projections

Different notations are used to represent the three-dimensional structure of molecules on two-dimensional surfaces. One of the most commonly used representations is the dash-wedge formula. The dashed wedges, solid wedges, and the plane lines indicate the groups situated behind the plane, coming out of the plane, and in the plane, respectively.
The organic molecules rotate across the single bonds leading to numerous temporary three-dimensional structures of varying energy known as conformers.
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Glycoconjugate diversification in <i>Campylobacter concisus</i> is determined by two glycosyltransferases.

bioRxiv : the preprint server for biology·2026
Same author

Detergent Exchange from Lipid Nanoparticles into Detergent Micelles Unlocks a Tool for Biochemical and Kinetic Characterization of Membrane Proteins.

Biochemistry·2026
Same author

Detergent exchange from lipid nanoparticles into detergent micelles unlocks a tool for biochemical and kinetic characterization of membrane proteins.

bioRxiv : the preprint server for biology·2026
Same author

Correlating membrane-protein dynamics with function: Integrating bioinformatics, molecular dynamics, and single-molecule FRET.

Protein science : a publication of the Protein Society·2025
Same author

Selection of nanobodies against liponanoparticle-embedded membrane proteins by yeast-surface display.

Protein science : a publication of the Protein Society·2025
Same author

Correlating membrane-protein dynamics with function: Integrating bioinformatics, molecular dynamics, and single-molecule FRET.

bioRxiv : the preprint server for biology·2025

関連する実験動画

Updated: Jul 13, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

マクロ分子結晶学構造の決定のための二重ランタニド結合タグ

Nicholas R Silvaggi1, Langdon J Martin, Harald Schwalbe

  • 1Department of Physiology and Biophysics, Boston University School of Medicine, 715 Albany Street, Boston, Massachusetts 02118, USA.

Journal of the American Chemical Society
|May 15, 2007
PubMed
まとめ

新しい二重ランタニド結合タグ (dLBT) は,X線結晶学を使用してタンパク質構造の決定を簡素化しています. この方法は複雑な修正を回避し,ユビキチンなどのタンパク質の段階化とモデル構築を容易にします.

さらに関連する動画

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
08:53

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Published on: October 2, 2017

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry
10:36

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry

Published on: June 15, 2021

関連する実験動画

Last Updated: Jul 13, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
08:53

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Published on: October 2, 2017

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry
10:36

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry

Published on: June 15, 2021

科学分野:

  • 構造生物学 構造生物学とは
  • バイオフィジックス 生物物理学
  • クリスタログラフィーです.

背景:

  • タンパク質の構造の決定は,生物学的機能を理解するために非常に重要です.
  • マクロ分子相化は,X線結晶学におけるボトルネックであり続けている.
  • 現在の方法は,しばしばタンパク質の誘導または非自然なアミノ酸を必要とします.

研究 の 目的:

  • X線結晶学におけるフェジングのための新しいペプチドタグを導入する.
  • 単一波長異常 difraktion (SAD) のためのダブルランタニド結合タグ (dLBT) の有用性を実証する.
  • ユビキチンなどのタンパク質の構造決定を容易にするため.

主な方法:

  • 高親和性ランタニド結合のための二重ランタニド結合タグ (dLBT) を設計しました.
  • ユビキチンとdLBTのN端融合構造を作成しました.
  • Tb3+イオンによる単一波長の異常微分法 (SAD) をフェージングに使用した.
  • 構造を完成させるための自動モデルの構築ソフトウェアを採用した.

主要な成果:

  • dLBTに結合したTb3+イオンを使用して,ユビキチンの相情報を決定しました.
  • 2.6 Åの解像度で明確な電子密度マップを取得しました.
  • 自動モデルの構築は,ユビキチン構造の約75%を成功裏に構築しました.
  • 不自然なアミノ酸や化学的改変の必要性を排除しました.

結論:

  • dLBTは,マクロ分子フェージングのための多用途なツールであり,既存の方法を補完します.
  • このテクニックは,特にタンパク質の誘導が困難である場合に,X線構造の決定を簡素化します.
  • dLBTは,フェーシングの広範な適用可能なソリューションを提供し,シンクロトロン設備への依存を潜在的に軽減します.