Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Phosphorylation01:02

Phosphorylation

The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
Phosphorylation01:02

Phosphorylation

The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Long-term Potentiation01:25

Long-term Potentiation

Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Hebbian LTP
LTP can occur when presynaptic neurons...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Worm-like gold nanocrystals fabricated using a self-assembling peptide and inducing cell death upon exposure to near infrared light.

Bioorganic & medicinal chemistry letters·2025
Same author

Intracellular evaluation of protein droplet-forming capability using self-assembling peptide tags.

Chemical science·2025
Same author

De novo designed YK peptides forming reversible amyloid for synthetic protein condensates in mammalian cells.

Nature communications·2024
Same author

Shape control of Au nanostructures using peptides for biotechnological applications.

Chemical communications (Cambridge, England)·2023
Same author

Proximity labeling and identification of endogenous client proteins recruited to Y15-based artificial granules tethering a bait protein.

Journal of peptide science : an official publication of the European Peptide Society·2023
Same author

Effect of linearly polarized microwaves on nanomorphology of calcium carbonate mineralization using peptides.

Scientific reports·2023

関連する実験動画

Updated: Jul 14, 2026

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
11:29

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents

Published on: September 4, 2015

リン酸媒介分子記憶は,情報入力要素として2つの異なるタンパク質キナーゼによって駆動されます.

Kin-Ya Tomizaki1, Hisakazu Mihara

  • 1Department of Bioengineering and The COE21 Program, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 B-40 Nagatsuta, Midori, Yokohama, Japan. tomizaki@rite.or.jp

Journal of the American Chemical Society
|June 15, 2007
PubMed
まとめ

研究者は,ブール論理演算を実行するペプチドを使用して分子記憶システムを開発しました. このシステムは,リン酸化と脱リン酸化を介して情報を記録,保存,読み取り,消去し,分子コンピューティングへの道を開く.

さらに関連する動画

Assembly and Characterization of Biomolecular Memristors Consisting of Ion Channel-doped Lipid Membranes
08:07

Assembly and Characterization of Biomolecular Memristors Consisting of Ion Channel-doped Lipid Membranes

Published on: March 9, 2019

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

関連する実験動画

Last Updated: Jul 14, 2026

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
11:29

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents

Published on: September 4, 2015

Assembly and Characterization of Biomolecular Memristors Consisting of Ion Channel-doped Lipid Membranes
08:07

Assembly and Characterization of Biomolecular Memristors Consisting of Ion Channel-doped Lipid Membranes

Published on: March 9, 2019

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

科学分野:

  • 分子工学は分子工学である.
  • バイオ分子コンピューティング
  • ナノテクノロジー ナノテクノロジー

背景:

  • ブル論理演算のための分子スケールの装置への関心が高まっている.
  • 以前の取り組みは,分子論理ゲートの化学的,物理的,生物学的入力に焦点を当てていました.
  • 情報処理のための堅牢な分子記憶システムが必要である.

研究 の 目的:

  • リン酸媒介分子記憶のための新しいタンデムタンパク質キナーゼ基板ペプチドの設計と合成.
  • この分子システムを使って基本的な論理演算 (AND,OR,NOR) を示します.
  • 分子ベースの情報処理とコンピューティングの可能性を調査する.

主な方法:

  • 2つのリン酸化可能な領域とN端のスピロピラン誘導体を持つタンデムペプチドの設計と合成.
  • 情報を記録するためにキナーゼ触媒によるリン酸化と,情報を消去するためにフォスファターゼ触媒による脱リン酸化を活用した.
  • 信号読み取りのためのスピロピランからメロシアニン (SPからMC) 熱着色変化と,論理関数をプログラミングするためのイオンポリマーを使用しています.

主要な成果:

  • リン酸化誘導によるSP-to-MC熱色変化に基づくAND,OR,およびNORの論理演算を成功裏に実証しました.
  • 情報は,フォスフォースターとして安定的に保存され,熱着色によって読み取られました.
  • システムは,記録,保存,読み出し,消去の完全なサイクルを実証しました.

結論:

  • 開発されたペプチドベースのシステムは,リン酸媒介分子メモリとして機能します.
  • 概念実証実験は,分子ベースの処理とコンピューティングの可能性を示しています.
  • この研究は,実用的な分子情報貯蔵および処理装置を開発するための基礎を提供します.