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RNA Structure01:23

RNA Structure

69.1K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
69.1K
RNA Stability01:53

RNA Stability

31.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
31.7K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

10.3K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
10.3K
Riboswitches01:56

Riboswitches

8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
RNA Structure01:19

RNA Structure

6.6K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
6.6K
Nucleic Acid Structure01:25

Nucleic Acid Structure

8.1K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
8.1K

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関連する実験動画

Updated: May 6, 2026

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

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バクテリアRNAポリメラーゼにおける基板負荷の構造的基礎

Dmitry G Vassylyev1, Marina N Vassylyeva, Jinwei Zhang

  • 1Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Schools of Medicine and Dentistry, 402B Kaul Genetics Building, 720 20th Street South, Birmingham, Alabama 35294, USA. dmitry@uab.edu

Nature
|June 22, 2007
PubMed
まとめ

RNAポリメラーゼのトリガーループ再折りメカニズムは,基板負荷に不可欠です. 構造的研究は,抗生物質開発のターゲットを提供する,明確なプレインセーションとインセーション状態を明らかにします.

さらに関連する動画

An Assay for Quantifying Protein-RNA Binding in Bacteria
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An Assay for Quantifying Protein-RNA Binding in Bacteria

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Antibody-Free Assay for RNA Methyltransferase Activity Analysis
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Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

6.8K

関連する実験動画

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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

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An Assay for Quantifying Protein-RNA Binding in Bacteria
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An Assay for Quantifying Protein-RNA Binding in Bacteria

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Antibody-Free Assay for RNA Methyltransferase Activity Analysis
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科学分野:

  • バイオケミストリー バイオケミストリー
  • 構造生物学 構造生物学とは
  • 分子生物学は分子生物学である.

背景:

  • RNAポリメラーゼ基板負荷を理解することは,転写の鍵です.
  • 正確なメカニズムは依然として議論の対象となっている.

研究 の 目的:

  • マルチサブユニットRNAポリメラーゼにおける基板負荷のメカニズムを解明する.
  • 基板結合と阻害の構造的基礎を決定する.

主な方法:

  • 3.0A解像度でのX線結晶学.
  • バイオケミカルアッセイ バイオケミカルアッセイ

主要な成果:

  • AMPcPPとAMPcPP/ストレプトリジジンによるThermus thermophilus延長複合体 (EC) の構造を決定した.
  • AMPcPPはトリガーループ (TL) リフォールディングを通じてアクティブな"挿入"部位に結合します.
  • ストレプトリジジンは,TL.を移動させることで,不活性な"前置置置"状態を安定させます.

結論:

  • TLの再折り畳みは,RNAポリメラーゼの触媒に不可欠です.
  • 基板負荷のための2段階のプレインセーション/インセーションメカニズムは,普遍的かもしれません.
  • 挿入前の状態は,新種の抗生物質の潜在的な標的である.