バクテリアのゲノム移植:種を別の種に変更する
Carole Lartigue1, John I Glass, Nina Alperovich
1J. Craig Venter Institute, Rockville, MD 20850, USA.
Science (New York, N.Y.)
|June 30, 2007
まとめ
科学者たちは,細菌のゲノムを別の種に移植し,合成ゲノムを持つ細胞を作り出した. 合成生物学におけるこの画期的な発見は,人工生命体を作るための道を切り開いている.
科学分野:
- 合成生物学 合成生物学とは
- 微生物ゲノミクス
- 細胞を再プログラムする.
背景:
- 細菌のゲノム移植は複雑なプロセスです.
- これまでの方法は,完全なゲノム置換を達成する上で限界があります.
研究 の 目的:
- 細菌細胞のゲノムをドナーのゲノムで完全に置き換えたことを実証する.
- 細胞を合成ゲノムで作る方法を確立する.
主な方法:
- 無傷のMycoplasma mycoides大コロニー (LC) のゲノムDNAをMycoplasma capricolum受容細胞に移植する.
- DNA配送のためのポリエチレングリコール媒介変換を使用しました.
- テトラサイクリン耐性のために選択され,成功したトランスフォーマーを特定します.
主要な成果:
- Mycoplasma mycoides LCの完全なゲノムを含む細菌細胞を成功裏に作成しました.
- 検出可能な受容者のゲノム配列を排除した結果の細胞.
- 移植されたゲノムは,ドナー株と同一のフェノタイプを与えました.
結論:
- ゲノム移植は,合成ゲノムを持つ細胞を作成するための有効な方法です.
- この技術は,合成ゲノムの人工的増殖に向けた重要な一歩を表しています.
- 結果となる細胞は,完全に置換されたゲノムの機能的生存性を実証しています.
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