血縁性マウス株の827万SNPの配列ベースのバリエーションマップ

Kelly A Frazer¹, Eleazar Eskin, Hyun Min Kang

¹Perlegen Sciences, 2021 Stierlin Court, Mountain View, California 94043, USA. frazer.kelly@scrippshealth.org

Nature

|July 31, 2007

まとめ

研究者は,実験用マウスの遺伝子変異をマッピングし,何百万もの単一ヌクレオチドポリモルフィズム (SNP) を特定しました. このマウスの詳細なゲノムマップは,マウスの進化と株の違いの理解を深める.

科学分野:

ゲノミクスゲノミクスとは
進化生物学の進化生物学について
比較ゲノミクスとは

背景:

実験用マウスの包括的な遺伝的多様性マップは,進化の歴史を理解するために不可欠です.
マウスの菌株における遺伝子型とフェノタイプの変化の関連性を調べることは,遺伝子研究にとって不可欠です.

研究の目的:

実験用マウスのゲノムにおける遺伝子変異の密度の高い地図を作成する.
Mus musculusの進化史についての洞察を得るために.
異なるマウス株におけるゲノタイプ-フェノタイプ関係の理解を向上させる.

主な方法:

野生産の4株と古典的な11株のマウスのゲノムを再配列した.
ゲノム全体にわたる高品質の単核酸ポリモルフィズム (SNP) を特定する.
特定されたSNPを使用してゲノム全体のハプロタイプマップを生成します.

主要な成果:

高品質の827万個の単核酸多形態 (SNP) を特定しました.
40,898のセグメントを持つゲノム全体のハプロタイプマップを生成しました.
古典的な株に対するMus musculus亜種の遺伝的貢献を決定し,M. m. domesticusが最も有意である.

結論:

密度の高いSNPマップは,マウスゲノミクスと進化論の研究に貴重なリソースを提供します.
亜種の貢献を理解することは,遺伝子型-現象型相関を解釈するのに役立ちます.
このデータは,他のマウス株における遺伝子型を割り当てるのを容易にし,多様性のより完全な見方を可能にします.

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