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Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
Bioreactor Controls-I01:28

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

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関連する実験動画

Updated: May 12, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 21, 2013

バクテリアの細胞サイズを制御する代謝センサー.

Richard B Weart1, Amy H Lee, An-Chun Chien

  • 1Department of Biology, Washington University, St. Louis, MO 63130, USA.

Cell
|July 31, 2007
PubMed
まとめ

栄養素レベルは,細胞分裂に栄養素の可用性を結合することによって,細菌の細胞サイズを制御します. バチルス・サブティリスの新発見された代謝センサーは,細胞が適切なサイズに達し,DNA複製を完了した後のみ分裂することを保証します.

科学分野:

  • 微生物学 微生物学とは
  • 細胞生物学 細胞生物学
  • バイオケミストリー バイオケミストリー

背景:

  • 栄養素の利用可能性は,単細胞生物のサイズに大きく影響し,富裕な媒体は,栄養素の少ない条件と比較して,より大きな細胞サイズを促進します.
  • 細胞メカニズムは,栄養のレベルを感知し,適切な細胞サイズを確保するために細胞分裂のタイミングを調節するために存在する必要があります.
  • 栄養状態に基づいて細胞サイズを調節する能力は,微生物の成長と生存に不可欠です.

研究 の 目的:

  • 栄養素の利用がバチルス・サブティリスの細胞分裂のタイミングを調節する分子機構を特定する.
  • 栄養状態と細胞分裂機構を結びつける新しい代謝センサーの特徴を明らかにする.

主な方法:

  • 細胞分裂部位におけるエフェクタータンパク質の栄養に依存した局所化を研究した.
  • FtsZ細胞分裂タンパク質の組み立てを阻害するエフェクターUgtPの役割を評価した.
  • 異なる成長率でFtsZリングと細胞長さの比率に対するセンサーの影響を分析した.

主要な成果:

  • Bacillus subtilis.の細胞分裂に栄養素の可用性を結びつける代謝センサーを特定しました.
  • 効果因子UgtPが栄養に依存した方法で分裂部位に局所することを発見しました.

さらに関連する動画

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection
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Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Published on: September 27, 2016

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation
11:18

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation

Published on: January 7, 2019

関連する実験動画

Last Updated: May 12, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 21, 2013

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection
10:50

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Published on: September 27, 2016

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation
11:18

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation

Published on: January 7, 2019

  • UgtPがFtsZの組み立てを阻害し,FtsZのリングと細胞の長さの比率を一定に保つのが実証された.

    • 結論:

    • 特定された代謝センサーは,バチルス・サブティリス細胞が適切な質量まで成長し,分裂前に染色体分離を完了することを保証します.
    • この栄養を感知するメカニズムは,成長速度に関係なく,一貫した細胞サイズを維持します.
    • 効果因子UgtPは,細胞分裂プロセスと栄養状態の結合において重要な役割を果たします.