抗血清がプロラクチン受容体への作用を阻害する

R P Shiu, H G Friesen

Science (New York, N.Y.)

|April 16, 1976

まとめ

プロラクチン受容体を標的とするギニア豚の抗体は,プロラクチン受容体をブロックした.

科学分野:

エンドクリノロジーエンドクリノロジー
分子生物学は分子生物学である.
細胞生物学細胞生物学

背景:

ホルモンの作用は,生理学的プロセスにとって極めて重要です.
ポリペプチドホルモンが細胞の反応を誘発する特定のメカニズムは,完全に解明されていません.
プロラクチンは,乳腺の機能を調節する重要なホルモンです.

研究の目的:

プロラクチンの作用を媒介するプロラクチン受容体の役割を調査する.
ポリペプチドホルモンシグナル伝達における膜受容体の必要性に関する直接的な証拠を提供すること.

主な方法:

特別にプロラクチン受容体を標的にするギニアピッグの抗血清を使用した.
抗血清が[125I]プロラクチンと膜受容体に結合する効果を評価した.
測定されたプロラクチン媒介の [3H] ルシンのカゼインと [14C] アミノアシドバター酸輸送への組み込み.
抗血清が[125I]インスリン結合およびインスリン媒介イベントに及ぼす影響を評価した.

主要な成果:

抗血清剤は,プロラクチン [125I] の受容体への結合を選択的に抑制した.
プロラクチン媒介によるカゼインとアミノ酸輸送の合成は,抗血清剤によって有意に抑制されました.
抗血清は,インスリン結合またはインスリン主導の細胞活動に作用せず,特異性を確認しました.

結論:

膜受容体は,プロラクチンなどのポリペプチドホルモンの生物学的作用を媒介する上で不可欠で必須の役割を果たします.
この研究は,ホルモン特異性膜受容体の機能的意義に対する直接的な実験的証拠を提供します.

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