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Updated: Jul 13, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
11:51

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

トリプトファン類のサイト固有の組み込みは,細菌細胞の再結合タンパク質に組み込まれています

Inchan Kwon1, David A Tirrell

  • 1Division of Chemistry and Chemical Engineering and Joseph J. Jacobs Institute for Molecular Engineering for Medicine, California Institute of Technology, Pasadena, California 91125, USA.

Journal of the American Chemical Society
|August 10, 2007
PubMed
まとめ

研究者らは酵母酵素を設計して,新しいトリプトファンアナログをタンパク質に組み込み,光タンパク質に新しいスペクトル特性を生み出した. これは,ユニークな機能を持つタンパク質を設計するためのツールキットを拡張します.

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PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

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Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System11:47

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

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