関連酵母種間の転写因子結合部位の相違

Anthony R Borneman¹, Tara A Gianoulis, Zhengdong D Zhang

¹Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA.

Science (New York, N.Y.)

|August 11, 2007

まとめ

酵母種間の遺伝子規制の違いは,転写因子結合部位の急速な進化を明らかにする. この差異は遺伝子の変化を大幅に上回り,種の進化を推進しています.

科学分野:

進化生物学の進化生物学について
分子生物学は分子生物学である.
遺伝学遺伝学とは

背景:

種間遺伝子調節の理解は,表型多様性と進化の説明の鍵である.
転写因子 (TF) は,遺伝子調節において重要な役割を果たします.

研究の目的:

酵母種間の転写因子結合部位の差異を調査する.
種多様性におけるTF結合部位の進化の役割を決定する.

主な方法:

クロマチン免疫プレシピテーション (ChIP) を用いて,その後DNAマイクロアレイ分析を行った.
シドホイファルレギュレータ Ste12 と Tec1 の結合部位は,Saccharomyces cerevisiae,S. mikatae,およびS. bayanusで分析されました.

主要な成果:

ほとんどのSte12とTec1結合部位は,研究された酵母種間で異なっています.
結合部位の差異は,オートロゴス遺伝子の種間差異を大幅に上回った.
Ste12標的のサブセットはS. mikataeとS. bayanusに独特に結合しており,経路の専門化を示唆しています.

結論:

転写因子結合部位は,正体遺伝子の内容よりもはるかに速く進化する.
TF結合による遺伝子調節の相違は,種間の進化的相違の主要な原動力である.

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