分子ノイズとサイズ制御が芽生えた酵母細胞サイクルにおける変動性に与える影響
Stefano Di Talia1, Jan M Skotheim, James M Bean
1The Rockefeller University, New York, New York 10021, USA.
Nature
|August 24, 2007
まとめ
分子騒音は,酵母における細胞サイクル精度に影響します. プライドと遺伝子投与は,変異性を減少させ,細胞サイクル進行を制御する異なるサイズ感知およびタイミングモジュールを明らかにします.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- システム生物学 システム生物学
背景:
- 遺伝子発現における分子騒音は,タンパク質濃度の変動を引き起こします.
- この騒音がユカリオット細胞サイクル制御の精度に与える影響は十分に理解されていません.
研究 の 目的:
- 芽生えた酵母細胞周期の精度に対する分子騒音の影響を調査する.
- 細胞サイズの貢献と細胞サイクル変動に対する他の要因を定量化するために.
主な方法:
- 光で標識された芽生えた酵母の単細胞イメージング.
- G1相の持続時間と細胞タンパク質含有量の測定.
- 異なるプロイディレベル (1N/2N/4N) と遺伝子用量における変動性の分析.
主要な成果:
- G1の変動性は,プロイディアとG1サイクリン遺伝子の投与量が増えるにつれて減少します.
- 細胞サイズ制御は,子細胞のG1変動に大きく寄与するが,母細胞には寄与しない.
- サイズに関係ないノイズは,子細胞でもG1の変動の主な要因です.
- G1段階は,サイズセンシング (CLN3-依存) とタイミング (CLN2-依存) の2つの独立したモジュールに分かれています.
結論:
- 分子騒音は,細胞サイクル精度に大きな影響を及ぼします.
- 細胞周期調節には,サイズセンシングとタイミングのための,別々の独立したモジュールが含まれています.
- G1サイクリン遺伝子のCLN3とCLN2は,これらの規制モジュールで異なる役割を果たしています.
さらに関連する動画
07:59Author Spotlight: Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons
Published on: June 9, 2023
2.0K
08:13Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
Published on: September 26, 2025
796
関連する概念動画
01:28Yeast Signaling
15.6K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
15.6K
02:11The Cell Cycle Control System
11.9K
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
11.9K
02:36Cells Coordinate Growth and Proliferation
3.8K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
3.8K
01:28The Cell Cycle Control System
5.1K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
5.1K
01:27Molecular Factors Affecting Cell Division
3.9K
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
3.9K
01:22Bioreactor Controls-III
67
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
67