テトラヒメナ・パイリフォーミスの凍結と活性は,ジメチル硫黄酸化物で
Science (New York, N.Y.)
|April 3, 1964
まとめ
Tetrahymena pyriformisのようなシリアドプロトゾアは,極端な寒さに耐えることができます. この研究では,2段階の冷凍プロセスと液体窒素貯蔵を使用して保存が成功し,運動性と繁殖を維持することが示されています.
科学分野:
- 微生物学 微生物学とは
- クリオバイオロジー クリオ生物学
- 細胞生物学 細胞生物学
背景:
- シリアド・プロトゾアは単細胞のエウカリオートである.
- 低温での微生物の保存は,研究とバイオテクノロジーにとって極めて重要です.
研究 の 目的:
- 糸状原生体の冷凍保存の可能性を調査する.
- Tetrahymena pyriformisが,冷凍後に生き残り,機能を維持できるかどうかを判断する.
主な方法:
- Tetrahymena pyriformisは,10%のジメチル硫酸化物 (冷凍保護剤) に суспенズされた.
- 2段階の冷凍プロトコルが採用されました: -20°C,その後すぐに -196°Cに移動します.
- サンプルは液体窒素の冷蔵庫に保存された.
主要な成果:
- Tetrahymena pyriformisは2段階の凍結プロセスに生き残った.
- 解凍後,原生体は回復した運動性を示した.
- また,プロトゾアの繁殖能力は,冷凍と保存後に回復しました.
結論:
- ディメチルスルフォキシドを使用した2段階の冷凍法が,Tetrahymena pyriformis.を冷凍保存するのに有効です.
- この技術により,毛糸状原生虫を液体窒素で長期にわたって保存し,その生存能力と生殖能力を維持できます.
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