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単一の遺伝子単位は,トウモロコシの要素アクティベーターの2つのトランスポーゼーション機能を指定します
Science (New York, N.Y.)
|October 10, 1986
まとめ
トウモロコシのアクティベーター (Ac) トランスポーザブル要素は,2つの遺伝的機能を通じて自身のトランスポーズを誘導し,抑制します. この2つの機能は,単一の遺伝子ユニットから発生し,完全な活動のために,オープン・リーディング・フレーム1と2を必要とします.
科学分野:
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
- 植物科学 植物科学について
背景:
- トウモロコシの移植可能な要素 アクティベーター (Ac) と ディソシエーション (Ds) は,移動性遺伝子配列である.
- Acは,Dsの転移を誘導し,投与量を増やして転移を阻害する二重の機能を発揮する.
- 以前の研究では,Ac要素内の3つのオープン・リーディングフレーム (ORF) が特定されました.
研究 の 目的:
- Acの二重機能 (転置誘導と投与量依存的阻害) の遺伝的基礎を調査する.
- これらの機能を媒介するAc要素内の特定のORFの役割を決定する.
- 異なるDs.要素の間の遺伝的補完性を理解する.
主な方法:
- AcおよびDs元素の誘導体 (wx-m9(Dsとbz-m2(DI)) を特定の削除で分析する.
- 転置回復を評価するための遺伝的補完測定法.
- AcのORF内の削除位置の特徴付け.
主要な成果:
- wx-m9(Ds) のORF 1での削除により,トランスポーゼーションが廃止されました.
- 主にORF 2で bz-m2 ((DI) を削除した結果,欠陥のある Ds 要素も発生した.
- wx-m9 ((Ds) と bz-m2 ((DI) は Acの転移機能を回復するために遺伝的に補完しなかった.
- bz-m2(DI) は,Acの阻害的な用量効果に寄与しなかった.
結論:
- ORF1とORF2は,ACの転置誘導機能のためにともに必要である.
- Acによる転置の誘導と阻害は,同じ遺伝的機能単位から生じる.
- この研究は,Acの転置可能な元素の調節の分子基礎を明らかにしています.
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