関連する実験動画

Updated: Jul 7, 2026

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
09:40

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Published on: August 26, 2010

高解像度電子顕微鏡およびシリコン上のネイティブ酸化物のスキャニングトンネル顕微鏡

A H Carim, M M Dovek, C F Quate

    Science (New York, N.Y.)
    |August 7, 1987
    PubMed
    まとめ

    先進顕微鏡を用いたシリコン原生酸化物構造の調査は,フッ化水素酸エッチングにより,硫黄過酸化物処理と比較して,より薄い酸化物層とより滑らかな表面が得られ,半導体加工に影響を与えることを明らかにしています.

    さらに関連する動画

    In Situ Characterization of Boehmite Particles in Water Using Liquid SEM11:59

    In Situ Characterization of Boehmite Particles in Water Using Liquid SEM

    Published on: September 27, 2017

    Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy09:21

    Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

    Published on: July 20, 2019

    関連する概念動画

    Imaging Biological Samples with Optical Microscopy01:18

    Imaging Biological Samples with Optical Microscopy

    Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
    In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
    Super-resolution Fluorescence Microscopy01:37

    Super-resolution Fluorescence Microscopy

    Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
    Overview of Electron Microscopy01:25

    Overview of Electron Microscopy

    The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
    Scanning Electron Microscopy01:07

    Scanning Electron Microscopy

    A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
    Fundamental Principles
    Accelerated...
    Preparation of Samples for Electron Microscopy01:20

    Preparation of Samples for Electron Microscopy

    To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
    Overview of Microscopy Techniques01:22

    Overview of Microscopy Techniques

    The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...