関連する実験動画
Updated: Jul 12, 2026
13:54
Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
Published on: April 2, 2013
細胞内バクテリアから多細胞性ユカリオットへの広範囲にわたる横向遺伝子転送
Julie C Dunning Hotopp1, Michael E Clark, Deodoro C S G Oliveira
1Institute for Genomic Research, J. Craig Venter Institute, 9712 Medical Center Drive, Rockville, MD 20850, USA. jhotopp@som.umaryland.edu
Science (New York, N.Y.)
|September 1, 2007
まとめ
細菌からユカリオットへの横向的な遺伝子転送は稀ですが,Wolbachiaのようなエンドシンビオントを通じて発生します. この研究は,昆虫およびネマトードゲノムへの遺伝子転送を確認し,真核生物における新しい遺伝子獲得の可能性を示しています.
科学分野:
- 微生物学 微生物学とは
- 遺伝学 遺伝学とは
- 進化生物学の進化生物学について
背景:
- ラテラル遺伝子転送 (LGT) は,細菌では一般的ですが,細菌と多細胞性エウカリオットでは稀です.
- ユーカリオットの生殖系に生息するWolbachia pipientisなどのエンドシンビオントは,宿主ゲノムへの細菌の遺伝子転送を媒介する可能性があります.
研究 の 目的:
- ヴォルバキア菌から真核宿主ゲノムへの遺伝子転送の証拠を調査する.
- これらの移植された遺伝子の範囲と機能を決定する.
主な方法:
- ウォルバキア・エンドシンビオンスを宿している昆虫とネマトード種のゲノム解析.
- 昆虫ゲノム配列の追加配列における潜在的な遺伝子転送イベントの計算式検出.
- 挿入されたバクテリアの遺伝子のための真核細胞の遺伝子転写の分析.
主要な成果:
- ウルバキアから4種の昆虫と4種のネマトドのゲノムに遺伝子の移転が確認された.
- 観察された挿入は,小さな断片から,ほぼWolbachiaの全ゲノムに及ぶ.
- さらに3つの昆虫ゲノムで潜在的転移が検出されました.
- 挿入されたウオルバキアのいくつかの遺伝子の転写が,エンドシンビオントなくとも,真核細胞に示された.
結論:
- プロカリオットシンビオントから真核宿主への遺伝性横行遺伝子転送が起こります.
- このプロセスは,エウカリウトが新しい遺伝子や機能を獲得するための潜在的なメカニズムを提供します.
- ヴォルバキアのエンドシンビオントは,宿主への遺伝子転送を促進する上で重要な役割を果たします.
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