アクチノリヒザル根の結節から細胞フリーな窒素酸および水素酸を採取する
Science (New York, N.Y.)
|August 17, 1979
まとめ
研究者らは,アルダー根の結節からアクティノミケート・エンドファイットを分離した. これらのエンドファイトは,窒素酵素と吸収ヒドロゲン酵素の活性を持つ細胞フリー製剤を産生し,その生物学的機能を実証した.
科学分野:
- 微生物学 微生物学とは
- 植物と微生物の相互作用
- バイオケミストリー バイオケミストリー
背景:
- アルダー (Alnus glutinosa) 植物は,根の結節にシンビオティックなアクティノミケートエンドファイットを宿している.
- これらのエンドファイトは宿主植物における窒素固定に不可欠である.
- これらのエンドファイトの酵素機構を理解することは不可欠です.
研究 の 目的:
- アクチノミケートエンドファイットの酵素的活動を,アルダー根の結節から分離し,特徴づけること.
- 細胞フリー製剤における窒素酵素と水素酵素酵素の存在と機能を調査する.
主な方法:
- 液体窒素中のアルダー根の結節の破壊.
- アクチノミケートエンドファイツの分離.
- 軽度の音波の振動により,エンドファイト細胞を分解する.
- 酵素測定のための細胞フリー抽出物の準備.
主要な成果:
- 窒素酶活性を含む細胞フリー製剤が得られた.
- この製剤はアセチレンと陽子を減少させる能力を示した.
- また,細胞フリー吸収ヒドロゲナーゼも同製剤で確認された.
結論:
- アルヌス・グルチノザの結節からのアクティノミケート・エンドファイットは,活性な窒素酶を持ち,水素酶酵素を吸収する.
- これらの酵素は抽出され,細胞のないシステムで活性を維持することができます.
- この研究は,シビオティックな窒素固定とアルダーエンドファイットの水素代謝のさらなる生化学研究のための基礎を提供します.
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