関連する実験動画

Updated: Jul 12, 2026

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

キラル化合物の電気運動分離

E Gassmann, J E Kuo, R N Zare

    Science (New York, N.Y.)
    |November 15, 1985
    PubMed
    まとめ

    この研究は,高電圧ゾーン電泳とレーザー光検出を使用して,ラセミアミノ酸のフェムトモール量を迅速に解消します. キラル分離法では,約10分で解析が可能である.

    さらに関連する動画

    Spatial Separation of Molecular Conformers and Clusters10:37

    Spatial Separation of Molecular Conformers and Clusters

    Published on: January 9, 2014

    On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids10:32

    On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

    Published on: March 2, 2012

    関連する概念動画

    Capillary Electrophoresis: Applications01:30

    Capillary Electrophoresis: Applications

    Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
    Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
    Electrophoresis: Overview01:20

    Electrophoresis: Overview

    Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
    There...
    Ion-Exchange Chromatography01:09

    Ion-Exchange Chromatography

    Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
    Capillary Electrophoresis: Instrumentation01:20

    Capillary Electrophoresis: Instrumentation

    Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
    Size-Exclusion Chromatography01:08

    Size-Exclusion Chromatography

    In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
    Silica particles offer advantages such as rigidity,...
    Racemic Mixtures and the Resolution of Enantiomers02:30

    Racemic Mixtures and the Resolution of Enantiomers

    A racemic mixture, or racemate, is an equimolar mixture of enantiomers of a molecule that can be separated using their unique interaction with chiral molecules or media. Racemic mixtures are denoted by the (±)- prefix. This ‘optical rotation descriptor’ applies to the whole solution of a racemic mixture rather than a specific stereoisomer. Enantiomers typically have the same physical and chemical properties. Hence, they are not easily separable. However, enantiomers can exhibit different...