関連する実験動画
Updated: Jul 12, 2026
11:42
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
Published on: April 7, 2017
まとめ
Rous サルコマウイルスはハムスターの細胞を変容させ,腫瘍の形成と成長の変化を引き起こします. いくつかの変形細胞は,非腫瘍原性の状態に戻り,正常な細胞の指向と移植能力を回復します.
科学分野:
- 腫瘍学 腫瘍学
- ウイルス学 ウイルス学 ウイルス学
- 細胞生物学 細胞生物学
背景:
- BHK-21細胞は,ウイルス変異研究のための標準的な細胞系である.
- ルース・サルコマウイルス (RSV) は,腫瘍を誘発することが知られているよく特徴づけられたレトロウイルスです.
研究 の 目的:
- Rous サルコマウイルス (Schmidt-Ruppin 株) によって BHK-21 細胞の変異を調査する.
- 変形した細胞の性質を特徴づけ,潜在的リバータント細胞系を特定する.
主な方法:
- BHK-21細胞をRousのサルコマウイルス (シュミット・ラッピン菌株) に感染させる.
- アガーサスペンション培養とガラス上の成長におけるコロニー形成の測定.
- ハムスターとニワトリにおける腫瘍起源性測定.
- 鶏における中和抗体の発達の分析.
- リバータント細胞クローンの分離と特徴付け.
主要な成果:
- トランスフォーメーションされたBHK-21細胞は,アンカレージ独立の成長 (アガー内のコロニー) とガラス上の無秩序な成長を示した.
- 変形細胞はハムスターやニワトリの腫瘍を誘発したが,細胞抽出物は腫瘍を誘発しなかった.
- 腫瘍を患った鶏は,RSVを中和する抗体を発達させた.
- リバータント細胞クローンは,ガラス上の組織的で並列の細胞指向を示し,アンカレージ独立の成長を失い,ハムスターの移植能力が低下した.
結論:
- Rous サルコマウイルスは,BHK-21細胞を効率的に変容させ,腫瘍の特性を誘発します.
- リバータント細胞系は,変形した細胞から生じ,正常細胞の特徴を取り戻すことができる.
- 腫瘍発生性とウイルス変異は,宿主の免疫反応と細胞の変化を含む複雑なプロセスです.
関連する概念動画
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
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