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Updated: Jul 11, 2026

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
10:39

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography

Published on: September 14, 2014

クリオエレクトロントモグラフィを用いたDNA表面活性剤単層の組織に関する洞察

Matthijn R J Vos1, Paul H H Bomans, Felix de Haas

  • 1Laboratory for Macromolecular and Organic Chemistry, Eindhoven University of Technology, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.

Journal of the American Chemical Society
|September 12, 2007
PubMed
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No abstract available in PubMed .

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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...