弾性組織工学のエスカフォルドのタンパク質プレコーティングは,細胞性を高め,細胞外マトリックスタンパク質の生産を強化し,循環する内皮原始細胞の現象型を差異的に調節しました

Virna L Sales¹, George C Engelmayr, John A Johnson

¹Department of Cardiac Surgery, Children's Hospital Boston, 300 Longwood Ave, Boston, MA 02115, USA. virna.sales@cardio.chboston.org

Circulation

|September 14, 2007

まとめ

心弁の支架を細胞外マトリックスタンパク質でプリコーティングすることで,内皮原始細胞の成長が著しく強化され,その行動が変化しました. このアプローチは,組織工学心臓弁のより良い制御を提供します.

科学分野:

バイオマテリアル科学バイオマテリアル科学
再生医学は,再生医療である.
組織工学は,組織工学である.

背景:

最適な細胞源と支架細胞の相互作用は,心臓弁の組織工学にとって非常に重要です.
本研究では,エンドセリアル原始細胞を用いたエラストメリックポリ (グリセロールセバケート) 構造物のタンパク質プレコーティングを調査しています.

研究の目的:

様々なタンパク質のプレコーティングが脚架細胞相互作用に与える影響を評価する.
プレコーティングが,内皮原始細胞の行動と細胞外マトリックス生成にどのように影響するかを決定する.

主な方法:

エラストメリックポリ (グリセロールセバケート) のスキャフォードは,ラミリン,フィブロネクチン,フィブリン,コラーゲンタイプI/III,またはエラスティンでプレコーティングされた.
羊の周周回血液内皮原生細胞を種まき,17日間培養した.
細胞の反応と機械的性質を分析した.

主要な成果:

タンパク質プレコーティングは,細胞性を有意に増加させ,内皮原始細胞のフェノタイプを変化させた.
細胞の行動と細胞外マトリックス生成の変化が観察されました.
種を蒔いた支架は,種を蒔かない対照群と比較して,効果的硬さが低下した.

結論:

細胞外マトリックスタンパク質でスキャフォルドをプレコーティングすることで,細胞の行動を正確に設計します.
この方法は,組織工学による心臓弁の構造の開発に価値があります.
細胞外マトリックス生産と細胞フェノタイプを変更することは,改良された構造の鍵です.

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