関連する実験動画
Updated: Jul 10, 2026
09:47
Analysis of Histone Antibody Specificity with Peptide Microarrays
Published on: August 1, 2017
JMJD6はヒストンアルギニン脱メチラーゼである
Bingsheng Chang1, Yue Chen, Yingming Zhao
1Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9038, USA.
Science (New York, N.Y.)
|October 20, 2007
まとめ
Jumonjiドメインを含む6タンパク質 (JMJD6) はヒストンH3およびH4アルギニン残基をデメチル化し,規制修正を逆転させます. この発見は,JMJD6ノックアウトマウスの発達障害を説明する可能性がある.
科学分野:
- バイオケミストリー バイオケミストリー
- 分子生物学は分子生物学である.
- エピジェネティクス エピジェネティクス
背景:
- アルギニンのメチル化は,様々な細胞機能を調節する.
- ヒストンアルギニンのメチル化 (モノメチル化と二メチル化) は,クロマチンの改造と遺伝子発現に影響を与えます.
- ヒストンアルギニンメチル化を逆転させる酵素は知られていません.
研究 の 目的:
- ヒストンアルギニンメチル化を逆転させることができる酵素を特定する.
- Jumonjiドメインを含む6タンパク質 (JMJD6) の酵素活性を特徴づける.
主な方法:
- JMJD6の酵素活性をテストするために生化学的測定法を使用しました.
- JMJD6の機能をin vivoで確認するために,細胞ベースのアッセイが使用されました.
- JMJD6は,JmjCを含む鉄および2オキシグルタレート依存型ダイオキシゲナーゼとして特定されました.
主要な成果:
- JMJD6は,ヒストンH3をアルギニン2 (H3R2) でデメチル化することが示されました.
- JMJD6は,アルギニン3 (H4R3) でヒストンH4をデメチル化することが示されました.
- これらの脱メチル化活動は,生化学的および細胞ベースのアッセイの両方で確認されました.
結論:
- JMJD6はヒストンアルギニン脱メチラーゼとして機能します.
- JMJD6の活動は,ヒストンアルギニンメチル化を逆転させるメカニズムを提供します.
- これらの発見は,JMJD6ノックアウトマウスで観察された発達障害の潜在的な説明を提供します.
関連する概念動画
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
02:30Histone Variants at the Centromere
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
02:25Spreading of Chromatin Modifications
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...
Writers
The writer is an enzyme that can...
02:54Nucleosome Remodeling
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
02:05Duplication of Chromatin Structure
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...