関連する実験動画
Updated: Jul 2, 2026

06:15
Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
Published on: August 18, 2018
BAI1は,ELMO/Dock180/Racモジュールの上流にあるアポプトシス細胞のエンゴルフメント受容体である
Daeho Park1, Annie-Carole Tosello-Trampont, Michael R Elliott
1Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908, USA.
Nature
|October 26, 2007
まとめ
脳特有の血管新生阻害剤1 (BAI1) は,フォスファティディルセリンをアポプトシス細胞に結合する受容体として作用する. BAI1はELMO/Dock180/Racモジュールを採用し,セルラー・デブリの効率的な吸収を媒介する.
科学分野:
- 細胞生物学 細胞生物学
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
背景:
- アポプトシス細胞のクリアランスは,多細胞生物にとって不可欠です.
- ELMO/Dock180/Racモジュールは,細胞死体を包み込む過程で,ファゴシートのアクチン細胞骨格を調節する.
- ELMO/Dock180/Racモジュールの上流の受容体は現在不明です.
研究 の 目的:
- ELMO/Dock180/Racのシグナリングモジュールの上流にある受容体を識別するために.
- アポプトティック細胞の吸収における脳特異性血管新生阻害剤1 (BAI1) の役割を調査する.
- BAI1のリガンドと細胞クリアランスのメカニズムを決定する.
主な方法:
- BAI1を新型エンゴルフメント受容体として特定.
- BAI1がフォスファティジルセリンに結合することを示すための生化学的分析.
- 浸食におけるBAI1の役割を評価するために,ex vivoおよびin vivoモデルを用いた機能的研究.
- 同免疫プレシピテーションは,ELMOとDock180.0.0とBAI1複合体の形成を示します.
主要な成果:
- BAI1は,アポプトシス細胞に発する"私を食べろ"信号であるフォスファティディルセリンと結合する受容体として特定されています.
- BAI1のトロンボスポンディン1型リピートは,直接のフォスファディチルセリン結合を媒介する.
- BAI1はELMOとDock180と共にコンプレックスを形成し,Rac-GEFモジュールと連携しています.
- BAI1機能の低下は,アポプトシス細胞の包囲を阻害する in vitro と in vivo.
結論:
- BAI1は,アポプトシス細胞の包囲に不可欠なフォスファティジルセリンの認識受容体として機能します.
- BAI1は直接ELMO/Dock180/Rac複合体を駆使して細胞のクリアランスを促進する.
- この発見は,エフェロサイトーシスの調節における重要な経路を明らかにしています.
関連する概念動画
Receptor-mediated Endocytosis
Overview
Receptor-mediated Endocytosis
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Receptor Downregulation in MVBs
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
The Extrinsic Apoptotic Pathway
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
The Intrinsic Apoptotic Pathway
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
Phagocytosis of Apoptotic Cells
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized by phagocytes.
Normal cells contain receptors that prevent them from being recognized by phagocytes.

