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Updated: Jul 10, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

オスミウム-DNAインターストランド複合体:ファシルDNAメチル化分析への応用

Kazuo Tanaka1, Kazuki Tainaka, Tadashi Umemoto

  • 1Frontier Research System, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama 351-1098, Japan.

Journal of the American Chemical Society
|October 30, 2007
PubMed
まとめ

研究者らは,DNAのシーケンス固有のオスミウム複合化のための新しい方法を開発した. このテクニックでは,機能化されたアデニン誘導体を用いて,DNAメチル化を検出し,定量化し,メチル化と非メチル化サイトシンを区別します.

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Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

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DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...