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関連する概念動画

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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関連する実験動画

Updated: May 10, 2026

Associated Chromosome Trap for Identifying Long-range DNA Interactions
14:49

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Published on: April 23, 2011

人間のCtIPは,DNAの末端解剖を促進する.

Alessandro A Sartori1, Claudia Lukas, Julia Coates

  • 1The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.

Nature
|October 30, 2007
PubMed
まとめ

人間のCtIPタンパク質は,DSB切除と同質再結合を促進することによって,DNA二重鎖断裂 (DSB) 修復に不可欠です. このタンパク質は,細胞サイクルチェックポイントのシグナル伝達に不可欠であり,その機能は種ごとに保たれています.

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Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
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Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

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関連する実験動画

Last Updated: May 10, 2026

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14:49

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Published on: April 23, 2011

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
11:11

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

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Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

科学分野:

  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学
  • 遺伝学 遺伝学とは

背景:

  • DNAの二重鎖断裂 (DSB) は,細胞サイクルチェックポイントのシグナル伝達と修復メカニズムを誘発する.
  • MRE11複合体は,DSB処理に関与することが知られている.

研究 の 目的:

  • DNA二重鎖断裂 (DSB) 修復におけるヒトCtIP (RBBP8) の役割を調査する.
  • CtIP,MRE11複合体,および同類再結合の関係を解明する.

主な方法:

  • CtIPのDSBへの募集の細胞サイクル分析.
  • DSBの解剖とRPA/ATRの採用におけるCtIPの役割の評価.
  • CtIP-MRE11相互作用を研究するための共免疫プレシピテーションアッセイ.
  • ホモローグな再結合アッセイ.
  • 酵母 Sae2 とのシーケンスホモロジー分析.

主要な成果:

  • CtIPは,DSB誘発剤に対する耐性を付与し,SとG2段階でDSBに徴用されます.
  • CtIPは,DSB切除に不可欠であり,RPAとATRの採用とATRの活性化につながる.
  • CtIPはMRE11複合体と物理的,機能的に相互作用する.
  • CtIPとMRE11の両方が,効率的な同類再結合のために必要である.
  • CtIPは酵母Sae2との配列ホモロジーを示し,機能が保存されていることを示唆しています.

結論:

  • CtIPは,DSB解剖,チェックポイントシグナル伝達,および同類再結合において重要な役割を果たします.
  • CtIPはMRE11複合体と協調して機能し,DSBの効率的な修理を可能にします.
  • CtIPのようなタンパク質は,DNA修復経路における進化的に保存された役割を持っています.