有機溶剤と互換性のある,ハイブリダイゼーション独立のDNAエンコード反応発見システムの開発と初期適用

Mary M Rozenman¹, Matthew W Kanan, David R Liu

¹Howard Hughes Medical Institute and the Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

Journal of the American Chemical Society

|November 13, 2007

まとめ

新しい化学反応を発見するためのDNAベースの方法を開発し,200以上の同時テストを可能にしました. このアプローチにより,黄金 (III) 触媒を用いた軽度で選択的な水酸化法が特定されました.

科学分野:

有機化学オーガニック・ケミストリー
化学生物学化学生物学とは
合成化学合成化学とは

背景:

DNAでコードされた化学は,反応の高通量スクリーニングを可能にします.
以前の方法は,しばしば特定のDNAハイブリッド化条件を必要とします.
金 (III) のような新しい触媒システムの探索は,反応の発見にとって極めて重要です.

研究の目的:

多用途のDNAエンコード反応発見プラットフォームを開発する.
このプラットフォームを金 (((III) 媒介型変換の探査に活用する.
ハイドロアリレーションのための新しい合成方法を特定する.

主な方法:

基板のペアリングとインビトロ選択のためのDNAエンコーディングを使用しました.
信号増幅のためにPCR増幅を使用した.
このシステムはDNAのハイブリッド化条件とは無関係に動作し,多様な反応環境を可能にします.
200以上の基板組み合わせを同時にスクリーニングしました.

主要な成果:

DNAでコードされた新しい反応発見アプローチを成功裏に開発し,適用しました.
シンプルで軽い金 ((III) 触媒化された方法が特定されました.
ヴィニルアレンと三置換オレフィンをインドールで選択的にマルコヴニコフ型水酸化させた.

結論:

開発されたDNAコード化されたシステムは,様々な条件下で反応発見のための強力なツールです.
このプラットフォームは,複雑な化学空間と触媒システムの探索を容易にする.
新しい選択的水酸化法により,有機化学における合成能力が拡大される.

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