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Updated: Jul 10, 2026

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
09:00

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

Published on: May 22, 2012

PCR増幅のための効率的な非自然なベースペア

Ichiro Hirao1, Tsuneo Mitsui, Michiko Kimoto

  • 1Protein Research Group, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan. ihirao@riken.jp

Journal of the American Chemical Society
|November 22, 2007
PubMed
まとめ

研究者らはDNA増幅のための新しい非自然な塩基対Ds-Pnを開発した. このエンジニアリングされた遺伝子システムは,PCRにおける高効率性と選択性を実証し,バイオテクノロジーのアプリケーションを拡大することを可能にします.

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RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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