関連する実験動画
Updated: May 8, 2026

10:52
Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
Published on: August 13, 2016
サイトプラズマのダイネインの力による双方向のステップアップ
Arne Gennerich1, Andrew P Carter, Samara L Reck-Peterson
1The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94158-2200, USA.
Cell
|November 30, 2007
まとめ
酵母細胞質ダイネインは8nmのステップで動きますが,力に対して逆行することができます. 驚くべきことに,ダイネインは核酸独立の運動を示し,運動調整に不可欠な力駆動による離離-結合サイクルを明らかにします.
科学分野:
- 細胞生物学 細胞生物学
- バイオフィジックス 生物物理学
- 分子モーターは分子モーターです.
背景:
- サイトプラズミックダイネインは,マイナスエンド方向のマイクロチューブルモーターである.
- 運動と力の発生の正確なメカニズムは完全に理解されていません.
研究 の 目的:
- 光学ピンチを使用して,酵母細胞質ダイネインの力依存のステップ動作を調査する.
- 過程的運動性におけるダイネインの運動領域の調整を解明する.
主な方法:
- 光学ピンチを使って,酵母細胞質ダイネインに制御された力を加えた.
- モータータンパク質のステップ行動と力に依存した運動性を分析した.
主要な成果:
- ダイネインは主に8nmのステップで移動し,4〜24nmのバリエーションがあります.
- 反対の力は後退のステップを増加させ,負荷>7 pNで逆転します.
- 負荷下での核酸独立の過程的ステップが実証され,力駆動サイクルを示しています.
結論:
- 力だけでは,ダイネインの脱離-結合サイクルを駆動することができます.
- これらの発見は,ダイネインにおける運動領域の調整のモデルを示唆しています.
- 細胞プロセスにおけるダイネインベースの運動性に関する新しい洞察を提供します.
関連する概念動画
Microtubule Associated Motor Proteins
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular cargos...
The Movement of Organelles and Vesicles
In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
Forces Acting on Chromosomes
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis.
Microtubules and motor proteins exert two types of forces on...
Microtubules and motor proteins exert two types of forces on...
Anaphase A and B
Microtubules form through the end-to-end polymerization of tubulin heterodimers. Kinetochore microtubules originate from the spindle poles, and their plus-ends connect with the kinetochores on sister-chromatids. Ndc80 protein complexes, present on the kinetochore, form low-affinity links with the plus end of these kinetochore microtubules.
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
Mechanism of Filopodia Formation
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Mechanism of Ciliary Motion
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...

