Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Intracellular Signaling Cascades01:24

Intracellular Signaling Cascades

Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
Intracellular Signaling Cascades01:24

Intracellular Signaling Cascades

Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
Amplifying Signals via Second Messengers01:15

Amplifying Signals via Second Messengers

Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Skeleton and Calcium Homeostasis01:21

Skeleton and Calcium Homeostasis

Calcium is not only the most abundant mineral in bone but also the most abundant mineral in the human body. Calcium ions are needed for bone mineralization, tooth health, heart rate regulation and strength of contraction, blood coagulation, the contraction of smooth and skeletal muscle cells, and the regulation of nerve impulse conduction. The average calcium level in the blood is about 10 mg/dL. When the body cannot maintain this level, a person will experience hypo or hypercalcemia.

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Phenotypic CRISPR screens identify NLRX1 as an essential activator of the human mitochondrial permeability transition.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Periodic ER-plasma membrane junctions support long-range Ca<sup>2+</sup> signal integration in dendrites.

Cell·2024
Same author

Elucidating and Optimizing the Photochemical Mechanism of Coumarin-Caged Tertiary Amines.

Journal of the American Chemical Society·2024
Same author

The SARS-CoV-2 accessory protein Orf3a is not an ion channel, but does interact with trafficking proteins.

eLife·2023
Same author

The SARS-CoV-2 accessory protein Orf3a is not an ion channel, but does interact with trafficking proteins.

bioRxiv : the preprint server for biology·2022
Same author

A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility.

Cell·2022

関連する実験動画

Updated: Jul 7, 2026

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)
09:32

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)

Published on: May 7, 2013

カルシウムシグナル伝達

David E Clapham1

  • 1Aldo R. Castañeda Professor of Cardiovascular Research, Professor of Neurobiology, Harvard Medical School, Howard Hughes Medical Institute, Enders 1309, Children's Hospital Boston, Boston, MA 02115, USA. dclapham@enders.tch.harvard.edu

Cell
|December 18, 2007
PubMed
まとめ
この要約は機械生成です。

カルシウムイオン (Ca2+) は細胞機能に不可欠であり,タンパク質の行動と細胞内レベルに影響を与えます. このレビューでは,Ca2+シグナル伝達,その局所的な作用,およびアポトーシスや転写などの細胞プロセスにおける役割について考察します.

さらに関連する動画

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
09:07

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators
08:01

Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators

Published on: January 19, 2024

関連する実験動画

Last Updated: Jul 7, 2026

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)
09:32

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)

Published on: May 7, 2013

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
09:07

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators
08:01

Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators

Published on: January 19, 2024

科学分野:

  • 細胞生物学 細胞生物学
  • バイオケミストリー バイオケミストリー
  • 生理学 生理学とは

背景:

  • カルシウムイオン (Ca2+) は,細胞内伝達物質である.
  • Ca2+は,膨大な数の細胞過程を調節する.

研究 の 目的:

  • Ca2+シグナル伝達の基本原理をレビューする.
  • サイトプラズマとオルゲンルのCa2+ホメオスタシスを制御するメカニズムについて議論する.
  • Ca2+信号伝導の局所的な性質を強調するために.

主な方法:

  • Ca2+シグナル伝達原理に関する文献レビュー.
  • タンパク質へのCa2+結合の検討.
  • Ca2+輸送とバッファリングシステムの分析.

主要な成果:

  • Ca2+結合はタンパク質の構造変化を誘導し,その機能を変化させます.
  • 細胞内Ca2+レベルを厳密に調節することは,細胞シグナル伝達に不可欠です.
  • Ca2+信号はしばしば高度に局所化され,特定の細胞反応を可能にします.

結論:

  • Ca2+は,多様な細胞機能における重要な調節剤として作用します.
  • Ca2+シグナル伝達を理解することは,細胞の興奮性,分泌,運動性,プログラム細胞死,遺伝子発現を理解する鍵です.