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関連する概念動画

Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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関連する実験動画

Updated: May 10, 2026

Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc
15:59

Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc

Published on: May 1, 2010

ドロソフィラのセグメンテーションにおける規制配列からの発現パターンの予測

Eran Segal1, Tali Raveh-Sadka, Mark Schroeder

  • 1Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel. eran@weizmann.ac.il

Nature
|January 4, 2008
PubMed
まとめ
この要約は機械生成です。

新しい熱力学モデルは,規制性DNA配列と転写因子の相互作用を分析することによって,メタゾウの発達における遺伝子発現パターンを正確に予測します. このコンピューティング・フレームワークは,発達の遺伝子ネットワークの理解を深める.

さらに関連する動画

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
09:45

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Published on: June 3, 2011

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
10:10

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

関連する実験動画

Last Updated: May 10, 2026

Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc
15:59

Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc

Published on: May 1, 2010

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
09:45

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Published on: June 3, 2011

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
10:10

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

科学分野:

  • 発達生物学 発達生物学とは
  • システム生物学 システム生物学
  • 計算生物学とは,計算生物学である.

背景:

  • 精密な空間時間的遺伝子発現は,メタゾアンの発達に極めて重要です.
  • 転写制御ネットワークのメカニズム的な理解は,まだ不完全である.

研究 の 目的:

  • 遺伝子発現パターンを予測するための新しい熱力学モデルを開発する.
  • シス調節配列と転写因子 (TF) がどのように開発をオーケストラ化するかを解明する.
  • このモデルをDrosophila melanogasterのセグメンテーション遺伝子ネットワークに適用する.

主な方法:

  • シス調節配列とTF結合/表現に基づく表現パターンを計算するための熱力学モデルを開発した.
  • このモデルをDrosophila melanogasterのシス調節モジュール (CRM) を分析するために適用した.
  • 強いTF結合サイトと弱いTF結合サイトの貢献とそのクラスタリングを調査した.

主要な成果:

  • このモデルは,CRMの表現パターンを正確に予測します.
  • ポジショナルの情報は,規制配列とTF分布でコード化されています.
  • 強い結合部位と弱い結合部位の両方が,高いDNA占有率と突然変異の強度に貢献します.
  • 弱い部位の短距離の同型クラスタリングは,協力的結合を可能にし,表現パターンを鋭くする.

結論:

  • 開発された熱力学モデルは,遺伝子調節を理解するための強力なツールを提供します.
  • 開発中のポジショナルの情報は,規制DNAとTFのランドスケープにコード化されています.
  • クラスタ化されたサイトを介して協力的な結合は,正確な開発パターンを設定するために不可欠です.