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関連する概念動画

Thermosensation01:43

Thermosensation

Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
Receptor Tyrosine Kinases01:26

Receptor Tyrosine Kinases

Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory organs,...

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関連する実験動画

Updated: May 11, 2026

Controllable Ion Channel Expression through Inducible Transient Transfection
10:00

Controllable Ion Channel Expression through Inducible Transient Transfection

Published on: February 17, 2017

細胞外チオレドキシンによるTRPCチャネル活性化

Shang-Zhong Xu1, Piruthivi Sukumar, Fanning Zeng

  • 1Institute of Membrane and Systems Biology, Garstang Building, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.

Nature
|January 4, 2008
PubMed
まとめ

細胞外チオレドキシンは,二酸化硫化物ブリッジを破ってTRPC5およびTRPC1イオンチャネルを活性化します. この新しく発見されたメカニズムは,チオレドキシンと細胞機能,特にリウマチ性関節炎を結びつける.

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Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
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Purification of Endogenous Drosophila Transient Receptor Potential Channels
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Purification of Endogenous Drosophila Transient Receptor Potential Channels

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関連する実験動画

Last Updated: May 11, 2026

Controllable Ion Channel Expression through Inducible Transient Transfection
10:00

Controllable Ion Channel Expression through Inducible Transient Transfection

Published on: February 17, 2017

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

Purification of Endogenous Drosophila Transient Receptor Potential Channels
08:39

Purification of Endogenous Drosophila Transient Receptor Potential Channels

Published on: December 28, 2021

科学分野:

  • イオンチャネル生物学
  • 分子細胞生物学 分子細胞生物学
  • レウマトロジーの病理学

背景:

  • 哺乳類の臨時受容体ポテンシャル (TRP) チャンネルは,細胞センサーとして機能します.
  • TRPチャネルの内生活性化物質と細胞機能を特定することは,重要な研究目標です.
  • 細胞外チオレドキシン (redoxタンパク質) は,大部分未知の細胞外標的を持っています.

研究 の 目的:

  • TRPC5とTRPC1チャネルの活性化メカニズムを調査する.
  • これらのチャネルを調節する細胞外チオレドキンの役割を決定する.
  • リウマチ性関節炎への影響を調査する.

主な方法:

  • チオレドキシンによるチャネル活性化を研究するための生化学的分析.
  • シノビオサイトにおけるTRPC5およびTRPC1発現の分析.
  • チャンネル遮断と分泌活動測定を含む機能研究.

主要な成果:

  • 減少したチオレドキシンがTRPC5ホモマルチメリックとTRPC5-TRPC1ヘテロマルチメリックチャネルを活性化します.
  • アクティベーションは,TRPC5.5の細胞外ループのディスルファイドブリッジの破壊によって発生します.
  • TRPC5とTRPC1は,関節リウマチ症のシノビオサイトで発現し,チオレドキシンによって活性化されます.
  • これらのチャネルを遮断すると,細胞の分泌活動に影響する.

結論:

  • 細胞外チオレドキシンを含む新しいイオンチャネル活性化メカニズムが特定されました.
  • このメカニズムは,細胞外チオレドキシンと細胞機能を結びつけ,リウマチ性関節炎に潜在的に関連しています.
  • TRPC5-TRPC1チャネルは,炎症性関節疾患の新たな治療標的である.