関連する実験動画
Updated: May 13, 2026
09:02
Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
Published on: December 10, 2015
Rumiは,Notchを改変するCAP10ドメインのグリコシルトランスフェラーゼであり,Notchのシグナル伝達には必要である
Melih Acar1, Hamed Jafar-Nejad, Hideyuki Takeuchi
1Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Cell
|February 5, 2008
まとめ
細胞運命を調節する遺伝子であるルミは,O-グルコシルトランスフェラーゼとして作用する. それは,タンパク質の折りたたみと細胞膜のシグナル伝達に影響を与える,エンドプラズマ網膜のO-グルコシレーティングノッチによってノッチシグナル伝達を変更します.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- ノッチ・シグナリングは,細胞運命を決定する上で極めて重要です.
- Notchシグナリングの調節を理解することは,発達生物学と疾患研究にとって不可欠です.
研究 の 目的:
- ノッチ・シグナル伝達の新しいレギュレータを特定し,特徴づけること.
- 特定された遺伝子であるルミがノッチ信号伝達に影響を与える分子メカニズムを解明する.
主な方法:
- 温度に敏感なミュータントスクリーンを利用して,Notchシグナル伝達に影響を与える遺伝子を特定しました.
- ルーミの標的と機能を決定するために,in vivo分析を行いました.
- ルーミの酵素活性を特徴付けるための生化学的分析.
主要な成果:
- 温度に敏感なNotchシグナル発信フェノタイプを持つ遺伝子として"rumi"が特定されました.
- ルーミはタンパク質O-グルコシルトランスフェラーゼ,特にO-グルコシル化ノッチとして作用します.
- ルーミは,エンドプラズマ網膜内のO-グルコシライティング・ノッチによって,ノッチの適切な折り畳みと取引を促進します.
結論:
- ルミは,ノッチタンパク質の成熟を調節することによって,ノッチの適切なシグナル伝達に不可欠です.
- エンドプラズマ網膜におけるノッチ・バイ・ルーミーのO-グルコシライゼーションは,細胞膜のシグナル伝達機能の重要なステップです.
関連する概念動画
01:56Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:16Calmodulin-dependent Signaling
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...