関連する実験動画
Updated: May 7, 2026
09:25
Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
Published on: June 4, 2014
神経上皮質幹細胞の増殖には,精密なスパインドル指向と対称的な分裂のためにLIS1が必要です
Jessica Yingling1, Yong Ha Youn, Dawn Darling
1Departments of Pediatrics and Medicine, Center for Human Genetics and Genomics, UCSD School of Medicine, 9500 Gilman Drive, La Jolla, CA 92098-0627, USA.
Cell
|February 13, 2008
まとめ
Lis1タンパク質は,発達中の脳細胞における細胞分裂指向の制御に不可欠である. この精密なスパインドルの方向性は,適切なニューロゲネシスを確保し,早期の神経幹細胞の細胞死を予防します.
科学分野:
- 神経科学は神経科学である.
- 発達生物学 発達生物学について
- 細胞生物学 細胞生物学
背景:
- ミトスのスパインドルの方向性は,哺乳類のニューロゲネシスにおける細胞分裂の結果を決定する.
- 初期の神経皮質幹細胞におけるスパインドル指向の役割は不明である.
- 放射性膠原原生細胞 (RGPCs) は,神経生成のために非対称な分裂を経験します.
研究 の 目的:
- 神経上皮質幹細胞とRGPCにおけるミトーシス・スパインドルの指向におけるLis1の役割を調査する.
- Lis1依存のスパインドル制御の基礎となる分子メカニズムを理解するために.
- Lis1欠乏が早期の神経幹細胞の増殖と生存に与える影響を決定する.
主な方法:
- マウスモデルにおけるLis1のインビボ制御された遺伝子削除.
- ミトスのスパインドル指向と細胞分裂パターンの分析.
- 細胞アポトーシスと増殖の評価.
- 微小管のダイナミクスとダイネインの局所化に関する研究.
主要な成果:
- Lis1は,神経皮質幹細胞とRGPCの両方の精密なミトーシススパインドル指向に不可欠です.
- 神経上皮質幹細胞のLis1欠乏は,対称的分裂の喪失によるアポトーシスにつながる.
- Lis1の喪失は皮質の微小管の捕獲を妨げ,微小管の安定性を低下させます.
- LISの結合パートナーであるNDEL1は,Lis1欠乏細胞におけるマイクロチューブルとダイネインの局所化を回復することができる.
結論:
- Lis1は,精密なスパインドル指向を通じて,神経上皮幹細胞における対称的な細胞分裂を調節する.
- LIS1/NDEL1/dynein複合体は,皮質の微小管捕獲に不可欠であり,適切なスパインドルの方向性を確保します.
- このメカニズムは,発達中の初期の神経幹細胞の増殖を維持するために不可欠です.
関連する概念動画
02:27The Mitotic Spindle
The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...
02:25Centrosome Duplication
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
02:50Spindle Assembly
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
02:19The Spindle Assembly Checkpoint
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
02:13Determining the Plane of Cell Division
Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function.
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division starting...
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division starting...
02:27The Mitotic Spindle
The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...