細胞サイクル初期に機能する分裂酵母B型サイクリン
A Bueno1, H Richardson, S I Reed
1Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Cell
|July 12, 1991
まとめ
新しく発見された分裂酵母遺伝子,cig1+は,新しいB型サイクリンをコードする. このサイクリンはG1からS段階への移行を調節し,これまで知られている細胞サイクルタンパク質とは異なる.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- サイクリンは細胞循環の重要な調節因子である.
- B型サイクリンは,典型的にはミトスの進行に関与する.
- 以前は,分裂酵母におけるcig1+の機能は知られていませんでした.
研究 の 目的:
- 分裂酵母遺伝子のシグ1+をクローンし,特徴づけること.
- 細胞サイクルにおけるcig1+タンパク質の役割を決定する.
- 他のサイクリンと比較してcig1+の機能的な特徴を調査する.
主な方法:
- 遺伝子クローニングとcig1+遺伝子の特徴化.
- 分裂酵母における消去と過剰表現の研究.
- 細胞サイクル進行と生命力の分析.
主要な成果:
- 分裂酵母遺伝子のcig1+は,B型サイクリンに類似した48 kDaのタンパク質をコードする.
- cig1+の削除は,G1からS段階の進行を大幅に遅らせます.
- cig1+の構成的過剰発現は致死性であり,G1の細胞を停止する.
- cig1+は,CLNサイクリンが欠けているS. cerevisiaeを救えない.
結論:
- cig1+は,B型サイクリンの一種である.
- このサイクリンは,G1からS段階への移行を調節する上で重要な役割を果たします.
- cig1+は,既知のミトシンおよびG1サイクリンとは機能的に異なる.
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