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関連する概念動画

Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Protein Denaturation01:28

Protein Denaturation

The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...

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Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
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Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

遺伝的にタンパク質をコードする酸化ダメージは,遺伝的にタンパク質をコードする酸化ダメージは,遺伝的にタンパク質をコードする酸化ダメージは,遺伝的にタンパク質をコードする酸化ダメージは,遺伝的にタンパク質をコードするタンパク質は,遺伝的

Heinz Neumann1, Jennifer L Hazen, John Weinstein

  • 1Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, United Kingdom.

Journal of the American Chemical Society
|March 7, 2008
PubMed
まとめ
この要約は機械生成です。

研究者は,3-ニトロチロジン (3-NT) を精密にタンパク質に挿入する方法を開発し,病気の研究を支援しました. この技術により,アルツハイマー病やパーキンソン病などの疾患において,タンパク質機能に対する3-NTの影響を研究することができます.

さらに関連する動画

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
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Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
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Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

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Last Updated: Jul 6, 2026

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
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Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

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Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
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Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

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科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • プロテイン工学は,タンパク質の

背景:

  • 3-ニトロチロシン (3-NT) を生成する翻訳後の改変は,神経変性疾患や癌を含む50以上の疾患に関連しています.
  • 老化組織における3NT濃度の上昇は,疾患リスクを予測し,その機能的影響を研究する必要性を強調しています.

研究 の 目的:

  • アミノアシル-tRNA合成酵素/tRNAペアをサイト特異で,タンパク質に3-NTを同翻訳的に組み込むために設計する.
  • 疾患モデルにおける特定の3-NT変異がタンパク質機能にどのように影響するかを調査するために,このシステムの有用性を実証する.

主な方法:

  • 新しいアミノアシル-tRNA合成酵素/tRNAペアの進化と特徴.
  • トランスレーション中のタンパク質にサイト固有の3-NTの組み込み.
  • 同型に窒素化されたマンガネス超酸化物ディスミュータゼ (MnSOD) を疾患に関連する部位で調製する.

主要な成果:

  • 遺伝子でコードされたサイト固有の3NT組み込みシステムの開発に成功しました.
  • 3NTで改変された同質的なMnSOD.を生産することによってシステムの有用性を実証.
  • MnSOD活動に対するサイト固有の窒素化の機能的影響の分析.

結論:

  • エンジニアリングされたアミノアシル-tRNA合成酵素/tRNAペアは,タンパク質の窒素化を正確に制御することを可能にします.
  • この方法は,様々な疾患状態における3 - NTの役割を研究するための強力なツールを提供します.
  • 神経学的障害,炎症,老化におけるタンパク質機能の変化に関する研究を容易にする.