ドロソフィラの進化的に保存されたサイクリンホモログは,G1サイクリンに欠けている酵母を救う
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Cell
|September 20, 1991
まとめ
研究者らは,酵母細胞の細胞循環機能を回復できる2つのドロソフィラ遺伝子を特定した. 1つの遺伝子はcdc2遺伝子のホモログであり,もう1つは酵母細胞サイズに影響を与える新しいサイクリンCタンパク質です.
科学分野:
- * 分子生物学 * 分子生物学
- * 遺伝学について
- * 細胞サイクル規制について
背景:
- * 細胞循環は真核細胞における基本的なプロセスで,サイクリンとサイクリン依存キナーゼ (CDK) によって調節される.
- *Saccharomyces cerevisiae (酵母菌) は,保存された遺伝経路により,細胞サイクル制御の研究のためのモデル生物として機能しています.
- *ドロソフィラ・メラノガスター (果物ハエ) は酵母と有意な遺伝的同質性を共有しており,比較研究のための貴重なツールとなっています.
研究 の 目的:
- * 細胞循環調節に関与するドロソフィラの遺伝子を特定し,特徴づけること.
- * 新型ドロソフィラ・サイクリン族の新種の機能を調査する.
- *サイクリン内の進化的に保存された領域と,他の腫瘍遺伝子との潜在的な同質性を調査する.
主な方法:
- *ドロソフィラのcDNAクローンの分離と特徴付け.
- * CLN機能に欠陥があるSaccharomyces cerevisiaeにおける機能的救済実験.
- * シーケンスホモロジーの分析とサイクリン間の保存領域の比較.
主要な成果:
- *2つのドロソフィラのcDNAクローンが分離され,CLN機能に欠陥のある酵母を救出しました.
- *1つのクローンは,cdc2遺伝子のドロソフィラホモログとして識別されました.
- *2番目のクローンは,ヒトの同種に非常に同型である新しいサイクリンCタンパク質をコードし,過剰発現すると異常に小さな酵母細胞サイズを誘導しました.
結論:
- *ドロソフィラは,cdc2.2のような重要な細胞サイクルレギュレータのホモログを持っています.
- * 新種のドロソフィラ・サイクリンCが特定され,酵母に機能的に保存され,細胞サイズに影響を与えています.
- *サイクリンに保存された領域は,ras oncogeneとの進化的リンクと潜在的な同質性を示唆しています.
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