ヒトサイクリンEは,CDC2遺伝子ファミリーの2つのメンバーと相互作用する新しいサイクリンです
1Department of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Cell
|September 20, 1991
まとめ
研究者らは,新しいヒトサイクリン,サイクリンEを特定し,CDC28タンパク質と相互作用して細胞サイクルSTARTを調節する. この発見は,ヒト細胞のG1からSへの移行を理解するために極めて重要です.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- 細胞循環の調節は,細胞増殖に不可欠です.
- サイクリンとサイクリン依存キナーゼ (CDK) は,細胞循環の重要な調節因子です.
- ヒトサイクリンの特定の役割を理解することは,細胞サイクル制御の解読に不可欠です.
研究 の 目的:
- 細胞循環調節に関与する新しいヒトサイクリンを特定し,特徴づけること.
- G1からS段階への移行における,新たに特定されたサイクリンEの機能を調査する.
- 細胞サイクル制御におけるヒトサイクリンEの相互作用パートナーを決定する.
主な方法:
- ヒトサイクリンを分離するためにSaccharomyces cerevisiaeのトリプルcln消去の補完.
- CDC28遺伝子との遺伝子相互作用の研究.
- E. coli. でのサイクリンEの発現と浄化
- ヒト細胞抽出物と抗体を用いたインビトロ結合およびキナーゼアッセイ.
- 免疫プレシピテーション研究.
主要な成果:
- 新しいヒトサイクリン,サイクリンEが分離されました.
- サイクリンEは,CDC28との遺伝的相互作用を示しており,START移行における役割を示している.
- 2つのヒトの遺伝子,CDC2-HSとCDK2のホモログが,サイクリンEの潜在的相互作用体として特定されました.
- ヒトG1細胞抽出物における再結合サイクリンE活性化CDC2タンパク質.
- サイクリンEに対する抗体は,HeLa細胞からヒストンH1キナーゼを免疫的に誘導した.
結論:
- ヒトのサイクリンEは,CDC28.28と相互作用することによって,細胞周期のSTARTで機能します.
- サイクリンEとCDC2またはCDK2の相互作用は,ヒト細胞のG1からS段階への移行に極めて重要です.
- サイクリンEは,ヒトの細胞サイクル制御機構の重要な構成要素です.
さらに関連する動画
10:33Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors
Published on: October 26, 2015
12:26Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
関連する概念動画
01:45Positive Regulator Molecules
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02:15M-Cdk Drives Transition Into Mitosis
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Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...