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ヘパトサイト成長因子としてのフィブロブラスト由来の上皮形状モルフォゲンの特定

R Montesano¹, K Matsumoto, T Nakamura

¹Department of Morphology, University Medical Center, Geneva, Switzerland.

|November 29, 1991

まとめ

フィブロブラスト由来肝細胞成長因子 (HGF) は,マディン・ダービー犬の腎臓細胞における上皮管形成を誘発する. この発見は,臓器発達の過程でパラクリン信号伝達と上皮質形態変異におけるHGFの役割を明らかにしています.

科学分野:

細胞生物学細胞生物学
発達生物学発達生物学とは
バイオケミストリーバイオケミストリー

背景:

マディン・ダービー犬の腎臓 (MDCK) 細胞は,標準的な条件下で in vitro で球状のキスタを形成します.
以前の研究では,線維芽細胞がMDCK細胞の分岐管の形成を誘導することを示しました.

研究の目的:

Epithelial tubulogenesisを誘導する責任を持つフィブロブラスト由来分子を特定するために.
肝臓細胞成長因子 (HGF) が表皮細胞形態変異における役割を調査する.

主な方法:

コラーゲンゲルでMDCK細胞を培養し,線維芽細胞/線維芽細胞条件付き媒介 (CM) を含むまたは含まない.
MDCKの細胞培養に外因的なHGFを加える.
HGFに対する抗体を用いて,線維細胞CMのチューボロゲン活性を中和する.

主要な成果:

MDCK細胞への外因的なHGF添加は,上皮管の形成を誘発した.
HGFに対する抗体は,フィブロブラストCMのチューボロゲン活性を完全に阻害しました.
フィブロブラスト由来のHGFは,上皮管形成の重要な媒介体である.

結論:

肝細胞成長因子 (HGF) は,上皮の形態変異のパラクリン媒介体として作用する.
HGFは,胚発達の過程でパレンキマ臓器の形成に重要な役割を果たします.
この研究では,HGFは上皮管の形成とオルガノゲネシスの重要な要因として特定されています.

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