B型サイクリンによるcdc25チロシンフォスファタゼの特異的活性化:ミトーシックサイクリンが複数の役割を果たす証拠
1Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, New York 11724.
Cell
|December 20, 1991
まとめ
2つの新しいヒトCDC25遺伝子,cdc25Aとcdc25Bが特定されました. それらはチロシンフォスファタゼを活性化し,細胞サイクル制御に不可欠なB型サイクリンによって調節されます.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- CDC25遺伝子産物は,細胞サイクル進行の重要な調節因子である.
- タイロシンリン酸化は,細胞サイクル制御において重要な役割を果たします.
研究 の 目的:
- ヒトの新型CDC25遺伝子を特定し,特徴づけること.
- CDC25チロシン・フォスファテーゼの活性に関する規制メカニズムを調査する.
主な方法:
- 分裂酵母変異体の遺伝子分離と機能的救済.
- ミトスの停止を研究するために,HeLa細胞に抗体のマイクロインジェクション.
- ティロシン・フォスファタゼの活性とタンパク質の相互作用を測定するためのインビトロアッセイ.
主要な成果:
- 2つの新しいヒトCDC25遺伝子であるcdc25Aとcdc25Bが分離され,機能的に特徴づけられました.
- cdc25Aとcdc25Bは,B型サイクリン (B1,B2) によって刺激されるチロシンフォスファタゼ活性を示すが,他のサイクリン型には刺激されない.
- HeLa細胞では,cdc25A,cyclin B1,cdc2との関連が観察されました.
- サイクリン相互作用の保存モチーフはチロシンフォスファタゼで特定されたが,cdc25には存在しなかった.
結論:
- B型サイクリンには多機能性があり,cdc2キナーゼ活性とcdc25チロシンフォスファターゼ活性の両方を調節します.
- cdc25Aとcdc25Bは,B型サイクリンによって活性化された細胞サイクル制御経路の重要な構成要素です.
- サイクリンBとの分子間相互作用は,おそらく,cdc25フォスファタゼに活性化ドメインを提供する.
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