クラトリンは,基礎側極性の重要な調節剤です

Sylvie Deborde¹, Emilie Perret, Diego Gravotta

¹Department of Ophthalmology, Dyson Vision Research Institute, LC-300, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10065, USA.

Nature

|April 11, 2008

まとめ

クラトリンは,上皮細胞における基礎側膜タンパク質の極性維持に不可欠である. この研究では,クラスリンが検出されました.

科学分野:

細胞生物学細胞生物学
分子生物学は分子生物学である.
バイオケミストリーバイオケミストリー

背景:

クラトリンで覆われた膀は細胞内交通を媒介し,エンドサイトーシスとシグナル伝達に不可欠です.
既知の非内皮細胞的役割には,ゴルギから溶解体ヒドローラゼ輸送が含まれています.
クラトリンの非内分泌的機能は,ほとんど未知のままである.

研究の目的:

ベース・ラテラルプラズマ膜タンパク質の極性におけるクラトリンの役割を調査する.
クラスリンがこれらのタンパク質の生合成配送とリサイクルに影響を与えるかどうかを決定する.
甲状腺細胞におけるタンパク質分類に対するクラトリンの影響を明らかにする.

主な方法:

利用したMDCKの上皮細胞系.
クラトリンのノックダウン実験 (慢性および急性) を実施しました.
タンパク質の密輸を追跡するために定量的なライブイメージングを使用しました.

主要な成果:

クラトリンのノックダウンにより,底側タンパク質の極性が乱され,配送とリサイクルの両方に影響を与えた.
アピカルタンパク質の極性は,クラトリンのノックダウンの影響を受けませんでした.
クラトリンの欠乏は,ゴルギから基礎側側タンパク質の排出を遅らせ,頂点の誤分類を促した.

結論:

クラトリンは,上皮細胞における基礎側タンパク質の密輸に広く必要とされています.
クラトリンは,上皮細胞の極性を維持する上で重要な役割を果たします.
これらの発見は,極化輸送におけるクラトリンの有意な非内分泌的機能を明らかにしています.

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