酵母菌におけるメオシス固有のRNAスプライシング
J A Engebrecht1, K Voelkel-Meiman, G S Roeder
1Department of Biology, Yale University, New Haven, Connecticut 06511-8112.
Cell
|September 20, 1991
まとめ
この研究は,酵母における微変の転写後の調節を明らかにしています. MER1遺伝子の産物は,MER2遺伝子のスプライシングに不可欠であり,メオシスの決定的なステップです.
科学分野:
- 分子生物学は分子生物学である.
- イースト遺伝学 イースト遺伝学
- セルラーレギュレーション セルラーレギュレーション
背景:
- 酵母におけるメオシスの調節は,以前は主に転写的と考えられていた.
- MER2遺伝子産物は,メオシスに不可欠である.
研究 の 目的:
- ミエオシスの転写後の調節を調査する.
- ミエオシスにおけるMER1遺伝子の役割を特定する.
主な方法:
- イーストにおけるMER2遺伝子転写とスプライシングの分析.
- MER1とMER2の遺伝子相互作用の遺伝子解析.
主要な成果:
- MER2はミトーシスと中間分裂の両方で転写されるが,中間分裂の間だけ効率的にスプライスされる.
- MER2のメイオティック・スプライシングは,MER1遺伝子産物に依存し,これはメイオティック細胞のみで生成される.
- MER1タンパク質は,MER2のトランスクリプトスプライシングを媒介する.
結論:
- 酵母におけるメオシスは,ポストトランスクリプションで調節される.
- MER1タンパク質は,メオシス中のMER2スプライシングに不可欠です.
- MER1は,他の中間遺伝子のスプライシングを調節する可能性があります.
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