ドロソフィラの背中腹部パターニング遺伝子トロイドは,ヒトの骨形態遺伝タンパク質1と関係しています
M J Shimell1, E L Ferguson, S R Childs
1Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
Cell
|November 1, 1991
まとめ
ドロソフィラトロイド (tld) 遺伝子の突然変異は,背側から腹側までのエクトダームの変化を引き起こします. トロロイド遺伝子は背部の発達に不可欠であり,デカペンタペリジック (dpp) と類似性を共有し,哺乳類に保存されています.
科学分野:
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
背景:
- ドロソフィラトロイド (tld) 遺伝子の変異により,部分的背外皮が腹外皮に変異する.
- tldのヌルフェノタイプは,背骨構造形成に不可欠なTGF-βファミリーのメンバーであるデカペンタプレジック (dpp) と比較できるが,より軽度である.
研究 の 目的:
- P要素タグ付けを使用して,tldロクスをクローンする.
- tld cDNAの配列を分析し,保存されたモチーフを特定する.
- 昆虫と哺乳類の間の信号伝達経路の進化的保存を調査する.
主な方法:
- TLDロクスをクローンするために,P要素タギングが使用されました.
- tldRNA発現は,ブラストダームの段階で分析されました.
- tld cDNA配列解析により,EGFのようなメタロプロテアゼと補完タンパク質の繰り返しが見つかりました.
主要な成果:
- tldRNAは,dpp.dpp.に似たように,ブラストダームの段階で背面に発現する.
- tldのcDNA配列は,メタロプロテアゼ,EGFのようなリピート,補完タンパク質とホモロジーを示しています.
- tldは,ヒトの骨形態遺伝タンパク質1 (BMP-1) と41%の配列同一性を共有しています.
結論:
- トロロイド遺伝子は,ドロソフィラの背中腹部のパターニングに重要な役割を果たしています.
- 特定された配列モチーフは,細胞外マトリックス改変またはシグナル伝達におけるtldの機能的役割を示唆しています.
- tldの進化的保存とBMPとの関係は,昆虫と哺乳類の発達におけるシグナル伝達経路が保存されていることを示唆しています.
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