Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...
Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Single-Particle Tracking of AMPA Receptor-Containing Vesicles.

Bio-protocol·2025
Same author

Activity-dependent synapse elimination requires caspase-3 activation.

eLife·2025
Same author

Transcriptional regulation of adipocyte lipolysis by IRF2BP2.

Science advances·2025
Same author

Transcriptional regulation of adipocyte lipolysis by IRF2BP2.

bioRxiv : the preprint server for biology·2024
Same author

Plasticity-induced actin polymerization in the dendritic shaft regulates intracellular AMPA receptor trafficking.

eLife·2024
Same author

Correction: Evolution of reduced co-activator dependence led to target expansion of a starvation response pathway.

eLife·2022

関連する実験動画

Updated: May 11, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

クロマチンはプロモーターの値をダイナミックレンジから切り離します.

Felix H Lam1, David J Steger, Erin K O'Shea

  • 1Howard Hughes Medical Institute, Department of Molecular and Cellular Biology, Faculty of Arts and Sciences Center for Systems Biology, Harvard University, 7 Divinity Avenue, Bauer 307, Cambridge, Massachusetts 02138, USA.

Nature
|April 18, 2008
PubMed
まとめ

核細胞は,誘導値とダイナミックレンジを分離することによって,遺伝子発現を制御する. プロモーターの変異は,結合部位の親和性とクロマチンの改造によって,環境信号への反応として遺伝子活性化を微調整する方法を示しています.

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • バイオケミストリー バイオケミストリー

背景:

  • クロマチンの構造,特に核子の位置付けは,DNAのアクセシビリティを制御することによって,遺伝子発現を調節する上で重要な役割を果たします.
  • Saccharomyces cerevisiaeでは,プロモーターは通常,トランスレーション開始部位の上流にあるヌクレオソームフリー領域 (NFR) を特徴とし,トランスクリプション因子が結合してクロマチンの改造を開始します.
  • 転写因子結合とクロマチンの組織が遺伝子発現の定量的な側面に影響を与える正確なメカニズムは,まだ完全に理解されていません.

研究 の 目的:

  • 核細胞と転写因子結合親和性が遺伝子誘導の値と発現のダイナミックレンジをどのように調節するかを調査する.
  • 変化する環境条件に応じて遺伝子発現を微調整するプロモーターアーキテクチャの役割を明らかにする.

主な方法:

  • Saccharomyces cerevisiaeにおける一連のプロモーター変異体の構築と分析.
  • 異なる生理学的刺激に反応する遺伝子活性化の定量評価.
  • 環境適応におけるプロモーター設計を理解するために,サッカロミセス・セレヴィセア・フォスファート (PHO) 反応経路の検討.

主要な成果:

  • 核細胞は,主に遺伝子誘導の値と発現のダイナミックレンジを切り離す機能を持っています.

さらに関連する動画

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

関連する実験動画

Last Updated: May 11, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

  • 転写因子結合部位の親和性は,遺伝子の活性化に必要な刺激のレベルに直接影響を及ぼします.
  • ヌクレオソーム領域内の結合部位は,染色体改造後の遺伝子発現レベルをスケーリングするのに寄与します.
  • PHO経路は,遺伝子発現を異なる環境のリン酸濃度に適応させるために,異なるプロモーター設計を使用します.

    • 結論:

    • 染色体構造と結合部位の親和の間の相互作用は,遺伝子発現反応を微調整するための洗練されたメカニズムを提供します.
    • これらの発見は,真核転写の定量制御に関する洞察を提供し,より詳細な転写モデルの開発を参考にすることができます.