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ヴァレンスの対位性により,z(*) 型イオンが化学的に区別される
Shane L Hubler1, April Jue, Jason Keith
1Department of Mathematics, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Journal of the American Chemical Society
|May 1, 2008
まとめ
新しいバレンスの対価ルールは,電子ベースのペプチド解離におけるz型イオンとc型イオンを区別する. この発見は,アミノ酸の組成を特定し,ペプチド配列を改良するのに役立ちます.
科学分野:
- プロテオミクス プロテオミクスは,プロテオミクスの
- 質量スペクトロメトリーによる質量スペクトロメトリーです.
- 化学分析 化学分析について
背景:
- 電子捕獲解離 (ECD) や電子移転解離 (ETD) などの電子ベースのペプチド解離法により,様々な産物イオンが生成されます.
- これらの製品イオンの化学組成を理解することは,正確なペプチド分析に不可欠です.
研究 の 目的:
- z (*) 型イオンの形成を制御する新しいバレンスの対価ルールを報告する.
- この規則がz (*) 型イオンと他の一般的な製品イオン,特にc型イオンをどのように区別するかを示します.
- ペプチド分析におけるこのルールの実用的な応用を示すために.
主な方法:
- 226 電子移転解離 (ETD) 産物イオンスペクトルの分析.
- 観測された産物イオンに化学組成物とイオン型を割り当てるために,バレンスの対価ルールを適用する.
- 規則の予測力の実験的検証.
主要な成果:
- z (*) 型イオンは,b型,c型,y型イオンとは異なり,奇数値を持つ偶数の原子を有する.
- 価率対数法則は,c型とz (*) 型イオンが同じ化学組成や質量を共有できないことを規定する.
- 観測されたc型とz型 (*) のイオンのほぼ半分は,m/zピークの単純な検査によって正しく割り当てられました.
結論:
- バレンスの対数法則は,c型とz型 (*) のイオンを区別するための簡単な方法を提供します.
- この規則は,アミノ酸組成の直接的な決定を容易にする.
- この発見は,データベース検索アルゴリズムを改良し,新たなペプチド配列決定の基礎となる.
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